Differential expression of Na+,K+-ATPase alpha- and beta-subunit mRNAs in rat tissues and cell lines.
Differential expression of Na+,K+-ATPase alpha- and beta-subunit mRNAs in rat tissues and cell lines.
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Na ,K -ATPase α 和 β 亚基 mRNA 在大鼠组织和细胞系中的差异表达。
DOI：
10.1073/pnas.84.24.9030
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发表时间：
1987
影响因子：
11.1
通讯作者：
Levenson,R
中科院分区：
文献类型：
--
作者：
Emanuel,JR;Garetz,S;Stone,L;Levenson,R
我们已经分析了Na+，K+-ATP酶（EC 3.6.1.3）α-和β-亚基mRNA在大鼠中枢神经系统来源的大鼠组织和细胞系中的表达。编码α亚基三种亚型的基因在组织和发育特异性表达方面存在显著差异。在所有测试组织中检测到α 1亚基基因的转录物，而α 2和α 3亚基mRNA主要在脑中表达。β-亚基mRNA的表达模式也是复杂的和组织特异性的，但不同于任何α-亚基mRNA。来自大鼠中枢神经系统和嗜铬细胞瘤PC 12的细胞系表达所有三种α亚基亚型的mRNA，而β亚基mRNA仅在PC 12细胞中检测到。大鼠Na+，K+-ATP酶mRNA的不同表达模式表明ATP酶家族的不同成员可能具有专门的功能。
We have analyzed Na+,K+-ATPase (EC 3.6.1.3) alpha- and beta-subunit mRNA expression in rat tissues and cell lines derived from the rat central nervous system. Substantial differences in the tissue and developmental specificity of expression were found for the genes encoding three isoforms of the alpha subunit. Transcripts of the alpha 1-subunit gene were detected in all tissues tested, whereas alpha 2- and alpha 3-subunit mRNA species were expressed predominantly in brain. The pattern of expression of beta-subunit mRNA also was complex and tissue specific but was distinct from that of any of the alpha-subunit mRNAs. Cell lines derived from the rat central nervous system and the pheochromocytoma PC12 expressed the mRNAs for all three alpha-subunit isoforms, whereas beta-subunit mRNA was detected only in PC12 cells. The distinct expression patterns of rat Na+,K+-ATPase mRNAs suggest that different members of the ATPase family may have specialized functions.