The effects of celecoxib on the proliferation and ultrastructural changes of MDA-MB-231 breast cancer cells
The effects of celecoxib on the proliferation and ultrastructural changes of MDA-MB-231 breast cancer cells
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DOI：
10.1080/01913123.2018.1459996
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发表时间：
2018-04
影响因子：
1
通讯作者：
Qing Ma;Yang Gao;Defei Wei;Nanyuan Jiang;L. Ding;Xin He;Lei Wei;Jingwei Zhang
中科院分区：
文献类型：
--
作者：
Qing Ma;Yang Gao;Defei Wei;Nanyuan Jiang;L. Ding;Xin He;Lei Wei;Jingwei Zhang
摘要：本研究旨在探讨塞来昔布对三阴性乳腺癌（TNBC）MDA-MB-231 细胞增殖和形态变化的影响。本研究采用一定浓度的塞来昔布处理MDA-MB-231细胞后，采用细胞计数试剂盒8（CCK-8）增殖实验检测细胞活力。 Western blotting用于分析凋亡相关蛋白caspase-3的表达水平。此外，用荧光和电子显微镜测定细胞和细胞核的形态变化。显微镜下观察细胞凋亡，细胞质空泡化。总的来说，塞来昔布可以通过增加 caspase-3 表达并引起超微结构变化来抑制 MDA-MB-231 细胞的增殖。
ABSTRACT This study sought to investigate the effects of celecoxib on the proliferation and morphological changes of triple-negative breast cancer (TNBC) MDA-MB-231 cells. In this study, after MDA-MB-231 cells were treated with a certain concentration of celecoxib, a cell counting kit-8 (CCK-8) proliferation assay was used to detect cell viability. Western blotting was utilized to analyze the expression level of caspase-3, which is an apoptosis-related protein. In addition, the morphological changes in the cells and nuclei were determined with fluorescence and electron microscope. Apoptotic nuclei and obvious cytoplasmic vacuolization were observed with a microscope. Collectively, celecoxib can inhibit the proliferation of MDA-MB-231 cells by increasing caspase-3 expression and causing ultrastructural changes.