Culture of mouse spermatogonial stem cells
Culture of mouse spermatogonial stem cells
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DOI：
10.1016/s0040-8166(98)80053-0
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发表时间：
1998-08-01
期刊：
影响因子：
2.6
通讯作者：
Brinster, RL
中科院分区：
文献类型：
--
作者：
Nagano, M;Avarbock, MR;Brinster, RL
精子发生发生在哺乳动物的曲细精管内，通过一个复杂的过程，是高度有组织的，非常有效的和非常多产的。这一过程的基础是精原干细胞，它能够自我更新和产生后代细胞，后代细胞经过数周至数月的分化，以产生成熟的精子。人们一直认为生殖细胞在培养中只能存活很短的时间，通常不到几周。然而，随着精原细胞移植技术的发展，准确评估精原干细胞在任何细胞群体中的存在只是最近才成为可能。使用这种技术，我们已经证明，小鼠精原干细胞可以保持在培养约4个月，并将产生精子发生后移植到一个适当的受体的曲细精管。在宿主体内产生广泛的培养供体细胞来源的精子发生区域，并产生成熟精子。睾丸细胞在STO饲养层上的培养有利于干细胞的存活。这些结果提供了第一步，在建立一个系统，将允许精原干细胞进行培养，并在体外增加其数量，以允许在移植到受体睾丸的遗传修饰。
Spermatogenesis occurs within the seminiferous tubules of mammals by a complex process that is highly organized, extremely efficient and very productive. At the foundation of this process is the spermatogonial stem cell that is capable of both self-renewal and production of progeny cells, which undergo differentiation over a period of weeks to months in order to generate mature spermatozoa. It had been thought that germ cells survive only a brief period in culture, generally less than a few weeks. However, an accurate assessment of the presence of spermatogonial stem cells in any cell population has only recently become possible with development of the spermatogonial transplantation technique. Using this technique, we have demonstrated that mouse spermatogonial stem cells can be maintained in culture for approximately 4 months and will generate spermatogenesis following transplantation to the seminiferous tubules of an appropriate recipient. Extensive areas of cultured donor cell-derived spermatogenesis are generated in the host, and production of mature spermatozoa occurs. Cultivation of the testis cells on STO feeders is beneficial to stem cell survival. These results provide the first step in establishing a system that will permit spermatogonial stem cells to be cultivated and their number increased in vitro to allow for genetic modification before transplantation to a recipient testis.