A distinct strain of Arsenophonus symbiont decreases insecticide resistance in its insect host.
A distinct strain of Arsenophonus symbiont decreases insecticide resistance in its insect host.
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DOI：
10.1371/journal.pgen.1007725
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发表时间：
2018-10
期刊：
影响因子：
4.5
通讯作者：
Zhang W
中科院分区：
文献类型：
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作者：
Pang R;Chen M;Yue L;Xing K;Li T;Kang K;Liang Z;Yuan L;Zhang W
共生细菌是昆虫表型多样性的重要驱动力。已出现的一种广泛存在的共生体属于阿森氏菌属，然而，它在大多数寄主昆虫中的生物学功能仍然完全不清楚。在这里，我们报道了褐飞虱(BPH)上的两个不同的砷磷菌株，褐飞虱是亚洲国家的一种主要害虫，通过对水稻作物的破坏造成了重大的经济损失。基因组重测序结果表明，S型毒蛾菌株对寄主的抗药性产生了负面影响。事实上，用S类型的毒鼠取代常驻的毒鼠显著降低了寄主对杀虫剂的抗性。转录组和代谢组分析表明，在S感染的白纹伊蚊宿主中，异源代谢下调，氨基酸积累增加。这项研究演示了共生体介导的表型变化是如何发生的。这项研究的结果将有助于制定通过对害虫施加生态劣势来发挥作用的策略，这将对农业生产中的害虫控制具有重要价值。共生细菌是关键的进化驱动力，提供创新，使它们的真核宿主进化出新的表型。通过获取特定共生体提供的有益特征已在许多种类的昆虫寄主中得到报道，然而共生体感染赋予昆虫寄主生态劣势的潜力却鲜有人关注。在这里，我们描述了一种新的共生关系，即食草性害虫褐飞虱与其兼性共生菌阿森氏菌之间的共生关系。我们在这种昆虫身上鉴定出两种不同的毒蛾菌株。一株以前已报道过的菌株(N型)，另一株很少观察到的菌株通过降低杀虫剂抗性而表现出相对敏感的作用(S型)。S型毒鼠的感染引起异种代谢的下调，这可能解释了致敏效应，同时反过来作为一种可能的权衡，增加了氨基酸在宿主中的积累。
Symbiotic bacteria are important drivers of phenotypic diversity in insects. One of the widespread symbionts to have emerged belongs to the genus Arsenophonus, however, its biological functions in most host insects remain entirely unknown. Here we report two distinct Arsenophonus strains in the brown planthopper (BPH), Nilaparvata lugens, a major pest insect in Asian countries that causes significant economic damage through rice crop destruction. Genomic resequencing data suggested that one Arsenophonus strain (S-type) negatively affected the insecticide resistance of the host. Indeed, replacement of the resident Arsenophonus with the S-type Arsenophonus significantly decreased host insecticide resistance. Transcriptome and metabolome analysis revealed down-regulation of xenobiotic metabolism and increased amino acid accumulation in the S-type Arsenophonus infected host. This study demonstrates how a symbiont-mediated phenotypic change can occur. The results of this study will aid in developing strategies that work through imposing an ecological disadvantage on insect pests, which will be of great value for pest control in agricultural industry. Symbiotic bacteria are key evolutionary drivers, providing innovations that allow their eukaryotic hosts to evolve new phenotypes. Beneficial traits provided by the acquisition of a specific symbiont have been reported in many kinds of insect host, however the potential of the symbiont infection to confer an ecological disadvantage to the insect host has received little attention. Here we describe a novel kind of symbiotic relationship between an herbivorous insect pest, Nilaparvata lugens, and its facultative symbiont Arsenophonus. We identified two distinct Arsenophonus strains in this insect. One strain had been previously reported (N-type), while the other, rarely observed strain presented a relatively sensitizing effect through reduction of insecticide resistance (S-type). Infection with the S-type Arsenophonus caused down-regulation of xenobiotic metabolism, which may explain the sensitized effect, while in turn serving to increase amino acid accumulation in the host as a putative trade-off.
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影响因子：
23.8
作者：
Douglas AE
通讯作者：
Douglas AE
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
DOI：
10.1073/pnas.1003379107
发表时间：
2010-07-06
影响因子：
11.1
作者：
Kirkness, Ewen F.;Haas, Brian J.;Pittendrigh, Barry R.
通讯作者：
Pittendrigh, Barry R.
DOI：
10.1098/rspb.2008.1476
发表时间：
2009-03-07
影响因子：
4.7
作者：
Guenduez, E. Akman;Douglas, A. E.
通讯作者：
Douglas, A. E.
使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
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