lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines
lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines
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lncRNA SNHG7通过下调葡萄膜黑色素瘤细胞系中的EZH2影响恶性肿瘤行为
DOI：
10.3892/ol.2019.11240
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发表时间：
2020-02-01
期刊：
影响因子：
2.9
通讯作者：
Zhang, Rui
中科院分区：
文献类型：
--
作者：
Wu, Xue;Yuan, Yiqun;Zhang, Rui
先前的研究表明，长非编码RNA，小核仁RNA宿主基因7（SNHG 7）在几种类型的癌症中起着重要的作用;然而，它在葡萄膜黑色素瘤（UM）的发展中的作用仍不清楚。本研究探讨SNHG 7对UM预后的影响，以及对UM细胞系的细胞增殖、细胞周期和凋亡的影响。此外，本研究旨在确定这些影响的分子机制。SNHG 7与UM预后之间的关联使用来自癌症基因组图谱数据库的详细SNHG 7 mRNA表达数据和临床信息进行分析。采用逆转录-定量PCR方法检测SNHG 7在UM组织和细胞系中的差异表达。在SNHG 7过表达后，使用细胞计数试剂盒-8测定法检测细胞增殖。使用碘化丙啶/RNA酶染色进行细胞周期测定。使用Annexin-V-异硫氰酸黄绿素细胞凋亡检测试剂盒进行细胞凋亡测定。通过蛋白质印迹法测量zeste增强子同源物2（EZH 2）的表达。本研究结果表明SNHG 7低表达与预后不良相关。此外，增加SNHG 7的表达可抑制UM细胞的增殖，抑制细胞周期进程，促进细胞凋亡。Western blot分析结果显示SNHG 7过表达下调UM细胞系中EZH 2蛋白的表达水平。本研究结果表明，SNHG 7通过调节EZH 2的表达抑制UM细胞的恶性转化。
Previous studies have demonstrated that the long non-coding RNA, small nucleolar RNA host gene 7 (SNHG7) plays an important role in several types of cancer; however, its role in the development of uveal melanoma (UM) remains unclear. The present study investigated the effect of SNHG7 on the prognosis of UM, as well as on cell proliferation, cell cycle and apoptosis of UM cell lines. Furthermore, the present study aimed to determine the molecular mechanisms underlying these effects. The association between SNHG7 and prognosis of UM was analyzed using detailed SNHG7 mRNA expression data and clinical information from The Cancer Genome Atlas database. Reverse transcription-quantitative PCR was used in order to detect the differential expression of SNHG7 in UM tissues and cell lines. Cell proliferation was detected using Cell Counting Kit-8 assays, following overexpression of SNHG7. A cell cycle assay was performed using propidium iodide/RNase staining. An apoptosis assay was performed using the Annexin-V-Fluorescein isothiocyanate apoptosis detection kit. The expression of enhancer of zeste homolog 2 (EZH2) was measured via western blotting. The results of the present study indicated that low expression of SNHG7 was associated with poor prognosis. Furthermore, increasing the expression of SNHG7 inhibited the proliferation of UM cells, suppressed cell cycle progression and promoted apoptosis. Western blot analysis results revealed that overexpression of SNHG7 downregulated EZH2 protein expression levels in UM cell lines. The results of the present study demonstrated that SNHG7 inhibited malignant transformation of UM cells by regulating EZH2 expression.