A survey of mutations in the Cochliomyia hominivorax (Diptera: Calliphoridae) esterase E3 gene associated with organophosphate resistance and the molecular identification of mutant alleles
A survey of mutations in the Cochliomyia hominivorax (Diptera: Calliphoridae) esterase E3 gene associated with organophosphate resistance and the molecular identification of mutant alleles
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DOI：
10.1016/j.vetpar.2006.04.010
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发表时间：
2006-09-10
影响因子：
2.6
通讯作者：
Lima de Azeredo-Espin, Ana Maria
中科院分区：
文献类型：
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作者：
Assis de Carvalho, Renato;Torres, Tatiana Teixeira;Lima de Azeredo-Espin, Ana Maria
Cochliomyia hominivorax（Calliphoridae）是最重要的引起蝇蛆病的蝇类之一，对整个新热带地区畜牧业造成严重的经济损失。在巴西，C. hominivorax 主要通过有机磷 (OP) 杀虫剂进行控制，尽管这些化学品的不当使用可能会导致产生抗性苍蝇。在某些节肢动物中，羧酸酯酶活性的变化与 OP 杀虫剂有关。在这项工作中，我们分离并表征了 C. hominivorax (Ch alpha E7) 中的部分 E3 基因，该基因包含与绿绿蝇 (Calliphoridae) 中获得 OP 水解酶活性相同的取代。用特异性识别突变位点的限制性内切酶消化聚合酶链式反应产物，明确区分野生和突变的酯酶等位基因。因此，PCR-RFLP 测定提供了一种快速、可靠的基于 DNA 的方法，用于鉴定具有酯酶基因突变的 C. hominivorax 个体。进一步的生物测定以确定这种突变与 C. hominivorax 的 OP 抗性之间的关系，应该可以开发出更有效的策略来管理该物种。 (c) 2006 Elsevier B.V. 保留所有权利。
Cochliomyia hominivorax (Calliphoridae) is one of the most important myiasis-causing flies and is responsible for severe economic losses to the livestock industry throughout the Neotropical region. In Brazil, C. hominivorax has been controlled mainly with organophosphate (OP) insecticides, although the inappropriate use of these chemicals can result in the selection of resistant flies. Changes in carboxylesterase activity have been associated with OP insecticides in some arthopodan species. In this work, we isolated and characterized part of the E3 gene in C. hominivorax (Ch alpha E7), which contained the same substitutions responsible for the acquisition of OP hydrolase activity in Lucilia cuprina (Calliphoridae). Digestion of the polymerase chain reaction products with a restriction enzyme that specifically recognized the mutation site unambiguously differentiated wild and mutated esterase alleles. The PCR-RFLP assay therefore provided a fast, reliable DNA-based method for identifying C. hominivorax individuals with a mutation in the esterase gene. Further bioassays to determine the association of this mutation with OP resistance in C. hominivorax should allow the development of more effective strategies for managing this species. (c) 2006 Elsevier B.V. All rights reserved.