Streptococcus koreensis sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion
Streptococcus koreensis sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion
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DOI：
10.1007/s00284-019-01778-6
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发表时间：
2019-12-01
影响因子：
2.6
通讯作者：
Kook, Joong-Ki
中科院分区：
文献类型：
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作者：
Lim, Yun Kyong;Park, Soon-Nang;Kook, Joong-Ki
从牙周炎患者龈下菌斑中分离到一株革兰氏阳性兼性厌氧球菌JS 71（T）。基于16 S rDNA基因的系统发育分析表明，该菌株属于链球菌属（Streptococcus）。16 SrDNA序列与Rubneri链球菌DSM 26920（T）、Streptococcus parasanguinis链球菌ATCC 15912（T）和Streptococcus australis链球菌CCUG 45919（T）的相似性分别为98.6%、98.5%和98.3%。菌株JS 71（T）的基因组长度为2，009，592 bp。DNA G+C含量为42.1mol%。菌株JS 71（T）与S. rubneri DSM 26920（T），S. parasanguinis ATCC 15912（T）和S. australisCCUG 45919（T）的转化率分别为88.9%、80.8%和92.4%。菌株JS 71（T）S. rubneri DSM 26920（T），S. parasanguinis ATCC 15912（T）和S. australisCCUG 45919（T）的抗性分别为36.5%（34-39%）、26.3%（23.9-28.7%）和48.0%（45.4-50.6%）。该菌株的主要脂肪酸为C-16：0（39.7%）、C-18：1 omega 6c/C-18：1 omega 7 c（15.5%）和C-18：0（10.4%）。根据这些结果，菌株JS 71（T）（= KCOM-2890（T）= JCM-33454（T））应该是链球菌属的一个新种，并建议将其命名为韩国链球菌（Streptococcus koreensis sp. nov.）。
A novel facultative anaerobic, Gram-stain-positive coccus, strain JS71(T), was isolated from the human subgingival dental plaque of a periodontitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) revealed that the strain belonged to the genus Streptococcus. The 16S rDNA sequence had high similarity with Streptococcus rubneri DSM 26920(T) (98.6%), Streptococcus parasanguinis ATCC 15912(T) (98.5%), and Streptococcus australis CCUG 45919(T) (98.3%). The genome of strain JS71(T) was 2,009,592 bp in length. The DNA G+C content of the strain was 42.1 mol%. Average nucleotide identity values between strain JS71(T) and S. rubneri DSM 26920(T), S. parasanguinis ATCC 15912(T), and S. australis CCUG 45919(T) were 88.9%, 80.8%, and 92.4%, respectively. Genome-to-genome distance values between strain JS71(T) S. rubneri DSM 26920(T), S. parasanguinis ATCC 15912(T), and S. australis CCUG 45919(T) were 36.5% (34-39%), 26.3% (23.9-28.7%), and 48.0% (45.4-50.6%), respectively. The major fatty acids of the strain were C-16:0 (39.7%), C-18:1 omega 6c/C-18:1 omega 7c (15.5%), and C-18:0 (10.4%). Based on these results, strain JS71(T) (= KCOM -2890(T) = JCM -33454(T)) should be a novel species of the genus Streptococcus, for which the name Streptococcus koreensis sp. nov. is proposed.