Regulated expression of the dinR and recA genes during competence development and SOS induction in Bacillus subtilis
Regulated expression of the dinR and recA genes during competence development and SOS induction in Bacillus subtilis
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DOI：
10.1111/j.1365-2958.1996.tb02657.x
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发表时间：
1996-10-01
影响因子：
3.6
通讯作者：
Hamoen, LW
中科院分区：
文献类型：
--
作者：
Haijema, BJ;vanSinderen, D;Hamoen, LW
已经假设枯草芽孢杆菌的dinR基因产物通过结合位于SOS基因上游的DNA序列（包括dinR和recA）而充当SOS调节子的阻遏物。由于DNA损伤而激活后，RecA被认为催化DinR-自切割，从而解抑制SOS调节子。目前的结果支持这一假设：dinR插入突变引起的高，组成型表达dinR和recA，这不能进一步提高SOS诱导。此外，凝胶阻滞试验表明dinR基因产物与recA和dinR启动子区域之间存在直接相互作用。上位性相互作用和凝胶阻滞试验表明，先前报道的recA的能力特异性表达直接依赖于comK，能力转录因子的基因产物。这些数据表明，存在着直接的监管联系之间的能力信号转导途径和SOS调节子。
It has been hypothesized that the dinR gene product of Bacillus subtilis acts as a repressor of the SOS regulon by binding to DNA sequences located upstream of SOS genes, including dinR and recA. Following activation as a result of DNA damage, RecA is believed to catalyse DinR-autocleavage, thus derepressing the SOS regulon. The present results support this hypothesis: a dinR insertion mutation caused a high, constitutive expression of both dinR and recA, which could not be further elevated by SOS-induction. In addition, gel-retardation assays demonstrated a direct interaction between the dinR gene product and the recA and dinR promoter regions. Epistatic interactions and gel-retardation assays demonstrated that the previously reported competence-specific expression of recA directly depended upon the gene product of comK, the competence transcription factor. These data demonstrate the existence of a direct regulatory link between the competence signal-transduction pathway and the SOS regulon.