Pseudomonas aeruginosa quorum-sensing systems may control virulence factor expression in the lungs of patients with cystic fibrosis
Pseudomonas aeruginosa quorum-sensing systems may control virulence factor expression in the lungs of patients with cystic fibrosis
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DOI：
10.1128/iai.70.4.1783-1790.2002
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发表时间：
2002-04-01
影响因子：
3.1
通讯作者：
Storey, DG
中科院分区：
文献类型：
--
作者：
Erickson, DL;Endersby, R;Storey, DG
患有囊性纤维化（CF）的个体通常定植有铜绿假单胞菌。由铜绿假单胞菌引起的慢性感染被肺部疾病的急性加重打断，这导致显著的发病率和死亡率。铜绿假单胞菌群体感应系统作为毒力决定因子的调节因子，可能在CF相关的慢性肺部感染中活跃。我们检测了CF患者肺中发现的细菌群体的自诱导分子和转录物积累水平。我们检测了CF患者痰液中N-（3-氧代十二烷酰基）-L-高丝氨酸（3-氧代-C12-HSL）和N-丁酰基-L-高丝氨酸内酯（C4-HSL）的生物活性水平。有趣的是，似乎在CF患者的肺中检测到C4-HSL的频率低于3-氧代-C12-HSL。我们还检查了自诱导物合酶基因lasI的转录，并表明它经常在CF患者的肺中表达。我们观察到Las quorum感应系统的last和四个靶基因的表达之间存在显著相关性。综上所述，我们的研究结果表明，群体感应系统是活跃的，并可能控制CF患者肺部的毒力因子表达。
Individuals with cystic fibrosis (CF) are commonly colonized with Pseudomonas aeruginosa. The chronic infections caused by P. aeruginosa are punctuated by acute exacerbations of the lung disease, which lead to significant morbidity and mortality. As regulators of virulence determinants, P. aeruginosa quorum-sensing systems may be active in the chronic lung infections associated with CF. We have examined the levels of autoinducer molecules and transcript accumulation from the bacterial populations found in the lungs of patients with CF. We detected biologically active levels of N-(3-oxododecanoyl)-L-homoserine (3-oxo-C12-HSL) and N-butyryl-L-homoserine lactone (C4-HSL) in sputum from CF patients. Interestingly, it appears that C4-HSL is less frequently detected than 3-oxo-C12-HSL in the lungs of patients with CF. We also examined the transcription of the autoinducer synthase gene lasI and showed that it is frequently expressed in the lungs of patients with CF. We observed a significant correlation between the expression of last and four target genes of the Las quorum-sensing system. Taken together, our results indicate that quorum-sensing systems are active and may control virulence factor expression in the lungs of patients with CF.