A comparison of the binding specificities of lectins from Ulex europaeus and Lotus tetragonolobus.
A comparison of the binding specificities of lectins from Ulex europaeus and Lotus tetragonolobus.
复制标题
欧洲豆科植物凝集素和四角莲凝集素的结合特异性的比较。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
K. Matta
中科院分区：
文献类型：
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作者：
H. Allen;E. Johnson;K. Matta
用亲和层析法从荆豆和百脉根的种子中分离到L-岩藻糖结合凝集素。通过血凝抑制分析比较了凝集素的结合特异性。凝集素的行为与各种合成的凝集素类似。D-岩藻糖衍生物作为抑制剂无活性。通常，α-L-吡喃岩藻糖基衍生物是比β-L-吡喃岩藻糖基衍生物更好的抑制剂。芳香糖苷配基增加了对硝基苯基-α-L-岩藻吡喃糖苷的抑制活性，是最好的抑制剂之一。两种凝集素之间的主要差异是观察到，与Lotus凝集素相比，Ulex凝集素需要更高浓度的β-内酰胺酶才能完全抑制血凝。
The L-fucose-binding lectins present in the seeds of Ulex europaeus and Lotus tetragonolobus have been isolated by affinity chromatography. The binding specificities of the lectins were compared by hemagglutination inhibition analysis. The lectins behaved similarly with respect to a variety of synthetic saccharides. D-Fucose derivatives were inactive as inhibitors. In general, the alpha -L-fucopyranosyl derivatives were better inhibitors than the beta-L-fucopyranosyl derivatives. Aromatic aglycones increased the inhibitory activity of the saccharides with p-nitrophenyl-alpha-L-fucopyranoside being one of the best inhibitors. The major difference between the two lectins was the observation that the Ulex lectin required higher concentrations of saccharides to bring about complete inhibition of hemagglutination than did the Lotus lectin.