Optically inducible membrane recruitment and signaling systems
Optically inducible membrane recruitment and signaling systems
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DOI：
10.1016/j.sbi.2019.01.017
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发表时间：
2019-08-01
影响因子：
6.8
通讯作者：
Chow, Brian Y.
中科院分区：
文献类型：
--
作者：
Hannanta-Anan, Pimkhuan;Glantz, Spencer T.;Chow, Brian Y.
通过膜相关蛋白和整体膜蛋白的光诱导细胞内信号传导，可以在时空上精确控制第二信使信号和细胞骨架重排，这对细胞迁移、发育和增殖很重要。光遗传膜募集感兴趣的蛋白，通过改变亚细胞定位来控制其信号是实现这些目的的一种通用手段。在这里，我们总结了RGS-LOV光感受器作为单组分膜募集工具的信号特征和潜在的结构功能，通过光调节与膜本身的静电相互作用，快速、可逆、有效地将蛋白质货物从细胞质运送到质膜。我们将这些最近描述的天然光感蛋白的技术相关特征放在总结蛋白质工程和光控膜蛋白信号的设计策略的背景下。
Optical induction of intracellular signaling by membrane associated and integral membrane proteins allows spatiotemporally precise control over second messenger signaling and cytoskeletal rearrangements that are important to cell migration, development, and proliferation. Optogenetic membrane recruitment of a protein-of-interest to control its signaling by altering subcellular localization is a versatile means to these ends. Here, we summarize the signaling characteristics and underlying structure-function of RGS-LOV photoreceptors as single-component membrane recruitment tools that rapidly, reversibly, and efficiently carry protein cargo from the cytoplasm to the plasma membrane by a light regulated electrostatic interaction with the membrane itself. We place the technology-relevant features of these recently described natural photosensory proteins in context of summarized protein engineering and design strategies for optically controlling membrane protein signaling.