Formation of cell-sized single-layered liposomes in a simple system of phospholipid, ethanol and water.
Formation of cell-sized single-layered liposomes in a simple system of phospholipid, ethanol and water.
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在磷脂、乙醇和水的简单系统中形成细胞大小的单层脂质体。
DOI：
10.1620/tjem.131.399
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
H. Sato
中科院分区：
文献类型：
--
作者：
K. Goto;H. Sato
将磷脂和水的乙醇溶液的混合物超声处理，在50℃下保持30分钟，然后用相差显微镜观察。由此获得的大量细胞大小的单层脂质体在形状和厚度上与通过常规方法制备的脂质体不同。用 La(NO3)3 和 KMnO4 固定可稳定脂质体。结果，它们在用于脱水和包埋的有机溶剂中保持不变。因此，可以用电子显微镜观察脂质体的超薄切片。讨论了这些脂质体的性质与细胞膜的结构和功能的关系。
A mixture of an ethanolic solution of phospholipid and water was sonicated, kept at 50 degrees C for 30 min, and then observed by a phase-contrast microscope. A large number of cell-sized, single-layered liposomes thus obtained were different in shape and thickness from the liposomes prepared by conventional methods. Fixation with La(NO3)3 and KMnO4 stabilized the liposomes. As a result they remained unchanged in the organic solvents used for dehydration and embedding. Ultrathin sections of the liposomes could, therefore, be observed with an electron microscope. The nature of these liposomes was discussed in relation to the structure and function of the cell membrane.