The luminal Ca2+ transient controls Ca2+ release/re-uptake of sarcoplasmic reticulum.
The luminal Ca2+ transient controls Ca2+ release/re-uptake of sarcoplasmic reticulum.
复制标题
DOI：
10.1006/bbrc.2000.4031
复制
发表时间：
2000-12
影响因子：
3.1
通讯作者：
N. Ikemoto;N. Ikemoto;Takeshi Yamamoto
中科院分区：
文献类型：
--
作者：
N. Ikemoto;N. Ikemoto;Takeshi Yamamoto
我们最近的研究（Saiki，Y.，和Ikemoto，N.，Biochemistry 38，3112-3119，1999）表明，Ca 2+释放和释放的Ca 2+的再摄取是协调的。以下结果表明，协调是由腔Ca 2+（[Ca 2 +]lum）瞬变介导的。在用多聚赖氨酸诱导被动负载的Ca 2+从SR释放后，腔Ca 2+（[Ca 2 +]lum）首先增加，然后降低（[Ca 2 +]lum瞬时）。在诱导Ca 2+释放后的不同时间监测SR Ca 2 + ATP酶的活性。磷酸酶（EP）的形成所确定的ANT-荧光增加的同时，在[Ca 2 +]lum的初始快速增加。EP衰减（泵营业额）加速同时减少的[Ca 2 +]流明。结果表明，[Ca 2 +]lum瞬变作为一个调解人的Ca 2+再摄取的加速后不久发生的Ca 2+释放的诱导。
Our recent study (Saiki, Y., and Ikemoto, N., Biochemistry 38, 3112-3119, 1999) suggests that Ca2+ release and re-uptake of the released Ca2+ are coordinated. The following results suggest that the coordination is mediated by the luminal Ca2+ ([Ca2+]lum) transient. Upon inducing the release of the passively loaded Ca2+ from the SR with polylysine, the luminal Ca2+ ([Ca2+]lum) first increased then decreased ([Ca2+]lum transient). The activity of the SR Ca2+ ATPase was monitored at different times after inducing Ca2+ release. The phosphoenzyme (EP) formation as determined by the MANT-fluorescence increased concurrently with the initial rapid increase in the [Ca2+]lum. EP decay (pumping turnover) was accelerated concurrently with a decrease of the [Ca2+]lum. The results suggest that the [Ca2+]lum transient serves as a mediator for the acceleration of the Ca2+ re-uptake occurring soon after the induction of Ca2+ release.