BMP-4 Extraction from Extracellular Matrix and Analysis of Heparin-Binding Properties.
BMP-4 Extraction from Extracellular Matrix and Analysis of Heparin-Binding Properties.
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DOI：
10.1007/s12033-021-00403-x
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发表时间：
2022-03
影响因子：
2.6
通讯作者：
Martinez-Hackert E
中科院分区：
文献类型：
--
作者：
Aykul S;Maust J;Martinez-Hackert E
重组人BMP-4生长因子（GF）作为骨再生治疗剂和细胞培养补充剂具有显著的商业潜力。然而，其商业用途受到限制，因为大规模表达和纯化人BMP-4 GF的尝试已被证明具有挑战性。我们已经建立了一种新的方法来获得大量的纯的和生物活性的BMP-4 GF从中国仓鼠卵巢细胞培养物中提取的GF部分从细胞外基质或细胞团部分。这种方法使产量比从CM纯化的BMP-4 GF增加约100倍。这两个馏分的分子活性是不可区分的。我们进一步分析了BMP-4 GF与蛋白聚糖肝素的结合，并表明N端碱性序列对这种相互作用是必不可少的。总之，这些结果提供了新的见解，纯化，定位，肝素结合的人BMP-4，其生物加工和生物功能的影响。
Recombinant human BMP-4 growth factor (GF) has significant commercial potential as therapeutic for regenerating bone and as cell culture supplement. However, its commercial utility has been limited as large-scale attempts to express and purify human BMP-4 GF have proved challenging. We have established a novel approach to obtain significant quantities of pure and bioactive BMP-4 GF from Chinese hamster ovary cell cultures by extracting the GF moiety from the extracellular matrix or cell pellet fraction. This approach increased yields approximately one 100-fold over BMP-4 GF purified from CM. The molecular activities of the two fractions are indistinguishable. We further analyzed binding of BMP-4 GF to the proteoglycan Heparin and showed that an N-terminal basic sequence is essential for this interaction. Taken together, these results provide novel insights into the purification, localization, and Heparin binding of human BMP-4 that have implications for its bioprocessing and biological function.
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DOI：
10.1016/j.btre.2018.e00249
发表时间：
2018-06
期刊：
Biotechnology reports (Amsterdam, Netherlands)
影响因子：
--
作者：
Gieseler GM;Ekramzadeh K;Nölle V;Malysheva S;Kempf H;Beutel S;Zweigerdt R;Martin U;Rinas U;Scheper T;Pepelanova I
通讯作者：
Pepelanova I
影响因子：
4.8
作者：
Nelsen, Sylvia M.;Christian, Jan L.
通讯作者：
Christian, Jan L.
密码子优化的人骨形态发生蛋白4在毕赤酵母中的表达
影响因子：
2.8
作者：
Huang, Yide;Zhen, Binqiong;Zhang, Yanding
通讯作者：
Zhang, Yanding
影响因子：
1.6
作者：
Swenckl-Underwood, Bethany;Mills, Juliane K.;Amegadzie, Bernard
通讯作者：
Amegadzie, Bernard
DOI：
10.1083/jcb.106.5.1659
发表时间：
1988-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lyons RM;Keski-Oja J;Moses HL
通讯作者：
Moses HL