Proliferation of immature plasma cells in pouchitis mucosa in patients with ulcerative colitis
Proliferation of immature plasma cells in pouchitis mucosa in patients with ulcerative colitis
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DOI：
10.1002/ibd.20447
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发表时间：
2008-08-01
影响因子：
4.9
通讯作者：
Arakawa, Tetsuo
中科院分区：
文献类型：
--
作者：
Hirata, Naoto;Oshitani, Nobuhide;Arakawa, Tetsuo
背景：结肠袋炎是溃疡性结肠炎（UC）患者恢复性直肠结肠切除术最常见的并发症。结肠袋炎的病因尚不清楚。我们以前曾报道过UC溃疡基底和炎症粘膜中未成熟浆系细胞的特异性和显著增殖。在本研究中，我们报告的结果回肠贮袋mucosal.Methods的表型研究：活检标本取自回肠贮袋的22例UC（12袋炎，10个正常的邮袋）和5例家族性腺瘤性息肉病（FAP）（与正常邮袋）谁进行了恢复性直肠结肠切除术，和正常回肠的10例UC尚未进行邮袋手术。从固定的样品中切下冷冻切片，并与各种淋巴细胞标记物和抗Ki-67抗体反应。结果：UC患者结肠袋炎黏膜中的Ki-67+细胞、CD 19+细胞和CD 138+细胞均显著增加，与UC患者的非炎症性结肠袋、FAP结肠袋和正常回肠相比，UC患者结肠袋炎黏膜中的CD 19 +Ki-67+细胞和CD 138 +Ki-67+细胞均显著增加。在炎症的贮袋粘膜中，CD 19 + CD 138+细胞的数量显著增加。我们观察到的CD 19 + CD 138+细胞数量的增加代表了未成熟浆细胞的增殖。此外，在CD 19细胞和CD 138细胞之间的Ki-67标记的增加表明这些细胞的增殖活性，与他们的immaturity.Conclusions一致：这些未成熟的浆细胞的增殖表明UC衍生的异常在结肠袋炎的发病机制中的参与的可能性。
Background: Pouchitis is the most common complication of restorative proctocolectomy in patients with ulcerative colitis (UC). The etiology of pouchitis is not known. We have previously reported the specific and significant proliferation of immature plasma lineage cells in the ulcer bases and inflamed mucosa of UC. In the present study we report the results of a phenotypic study of ileal pouch mucosa.Methods: Biopsy samples were taken from the ileal pouch of 22 patients with UC (12 with pouchitis, 10 with a normal pouch) and 5 patients with familial adenomatous polyposis (FAP) (with a normal pouch) who underwent restorative proctocolectomy, and normal ileum of 10 patients with UC yet to undergo pouch surgery. Frozen sections were cut from fixed samples and reacted with various lymphocyte markers and anti-Ki-67 antibodies. Ki-67+ cells, CD19+ cells, and CD138+ cells were significantly increased in the pouchitis mucosa of patients with UC.Results: Immunological double staining revealed significantly increased numbers of CD19+Ki-67+ cells and CD138+Ki-67+ cells in the pouchitis mucosa of patients with UC compared to noninflamed UC pouch, FAP pouch, and normal ileum of UC patients. The number of CD19+CD138+ cells was significantly increased in inflamed pouch mucosa. The increased number of CD19+CD138+ cells we observed represents proliferation of immature plasma cells. Moreover, the increase in labeling for Ki-67 among CD 19 cells and CD 138 cells suggests proliferative activity of these cells, consistent with their immaturity.Conclusions: Proliferation of these immature plasma cells suggests the possibility of involvement of UC-derived abnormality in the pathogenesis of pouchitis.