The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes
The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes
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DOI：
10.1016/j.molbiopara.2004.11.004
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发表时间：
2005-02-01
影响因子：
1.5
通讯作者：
Clayton, C
中科院分区：
文献类型：
--
作者：
Brems, S;Guilbride, DL;Clayton, C
我们描述了布氏锥虫的两个菌株的发育调控基因：单形菌株李斯特427和多形菌株TREU 927。使用24，567个基因组片段的阵列获得表达模式。探针由血流形式或顺循环形式的锥虫制备。14个顺周期特异性和77个血流特异性信号从匹配变体表面糖蛋白或相关基因的序列中获得，另外17个调节序列是重复的或与转座元件相关的。286个受调控的点对应于来自其他蛋白质编码基因的mRNA;这些点代表191种不同的蛋白质。113个不同基因（79个来自前循环形式，34个来自血流形式）的调节得到至少两个独立实验或标准的支持：其中约60个是新的。只有两个基因-编码HSP 83和一个输入相关蛋白-似乎只在TREU 927菌株中受到调节。我们的研究结果证实了以前的估计，2%的锥虫基因在mRNA水平上显示发育调控。(C)2004 Elsevier B. V.保留所有权利。
We describe developmentally regulated genes in two strains of Trypanosoma brucei: the monomorphic strain Lister 427 and the pleomorphic strain TREU927. Expression patterns were obtained using an array of 24,567 genomic fragments. Probes were prepared from bloodstream-form or procyclic-form trypanosomes. Fourteen procyclic-specific and 77 bloodstream-specific signals were obtained from sequences matching variant surface glycoprotein or associated genes, and a further 17 regulated sequences were repetitive or transposable-element-related. Two hundred and eighty-six regulated spots corresponded to mRNAs from other protein-coding genes; these spots represent 191 different proteins. Regulation of 113 different genes (79 from procyclic forms, 34 from bloodstream-forms) was supported by at least two independent experiments or criteria: of these, about 60 were novel. Only two genes - encoding HSP83 and an importin-related protein - appeared to be regulated in the TREU927 strain only. Our results confirmed previous estimates that 2% of trypanosome genes show developmental regulation at the mRNA level. (C) 2004 Elsevier B.V. All rights reserved.