Phylogenetic analysis of H1N2 isolates of influenza A virus from pigs in the United States
Phylogenetic analysis of H1N2 isolates of influenza A virus from pigs in the United States
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DOI：
10.1016/s0168-1702(02)00053-9
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发表时间：
2002-08-01
期刊：
影响因子：
5
通讯作者：
Joo, HS
中科院分区：
文献类型：
--
作者：
Choi, YK;Goyal, SM;Joo, HS
美国从猪身上新分离出24株H1 N2甲型流感病毒。这些分离株来自1999年至2001年期间9个不同养猪州的19个农场。所有农场都有呼吸系统问题和/或流产的临床病史。对病毒分离株进行了遗传学表征，以确定所有8个基因片段的来源。结果表明，所有分离株均为H1N1和H3 N2三重弱毒株。H1 N2分离株的神经氨酸酶（NA）和PB 1基因是人源的，而血凝素（HA）、核蛋白（NP）、基质（M）、非结构（NS）、PA和PB 2聚合酶基因是禽源或猪源的。所有H1 N2分离株中有15株与美国第一株H1 N2分离株（A/SW/IN/9 K 035/99）具有密切的亲缘关系和高度的氨基酸同源性。其余9株与H1N1流感病毒HA基因同源性较高。其它基因NA、M、NP、NS、PA、PB 1和PB 2与H1 N2（A/SW/IN/9 K 035/99）株和H3 N2三重毒株的同源性均较低。而A/SW/KS/13481-S/00、A/SW/KS/13481-T/00两株分离株的PB 1基因来源于A型禽流感病毒谱系。这些结果表明，尽管HA基因存在一些变异，但在美国猪群中流行的H1 N2病毒具有相似的遗传谱系。(C)2002 Elsevier Science B. V.保留所有权利。
Twenty-four H1N2 influenza A viruses were newly isolated from pigs in the United States. These isolates originated from 19 farms in 9 different swine producing states between 1999 and 2001. All farms had clinical histories of respiratory problem and/or abortion. The viral isolates were characterized genetically to determine the origin of all eight gene segments. The results showed that all H1N2 isolates were reassortants of classical swine H1N1 and triple reassortant H3N2 viruses. The neuraminidase (NA) and PB1 genes of the H1N2 isolates were of human origin, while the hemagglutinin (HA), nucleoprotein (NP), matrix (M), non-structural (NS), PA and PB2 polymerase genes were of avian or swine origin. Fifteen of the all H1N2 isolates were shown to have a close phylogenic relationship and high amino acid homology with the first US isolate of H1N2 (A/SW/IN/9K035/99). The remaining nine isolates had a close phylogenic relationship with classical swine influenza H1N1 in the HA gene. All other genes including NA, M, NP, NS, PA, PB1 and PB2 showed a close phylogenic relationship with the H1N2 (A/SW/IN/9K035/99) strain and triple reassortant H3N2 viruses. However, PB1 genes of two isolates (A/SW/KS/13481-S/00, A/SW/KS/13481-T/00) were originated from avian influenza A virus lineage. These results suggest that although there are some variations in the HA genes, the H1N2 viruses prevalent in the US swine population are of a similar genetic lineage. (C) 2002 Elsevier Science B.V. All rights reserved.