Mitigation of experimental inflammatory bowel disease in guinea pigs by selective elimination of the aerobic gram-negative intestinal microflora.
Mitigation of experimental inflammatory bowel disease in guinea pigs by selective elimination of the aerobic gram-negative intestinal microflora.
复制标题
通过选择性消除需氧革兰氏阴性肠道微生物群来减轻豚鼠实验性炎症性肠病。
DOI：
10.1016/s0016-5085(19)32848-3
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发表时间：
1974
期刊：
影响因子：
29.4
通讯作者：
M. Anver
中科院分区：
文献类型：
--
作者：
D. V. D. Waaij;Bennett J. Cohen;M. Anver
通过选择性消除需氧革兰氏阴性肠道菌群，可以显着减轻豚鼠实验诱发的大肠溃疡病。在饮用水中添加 2% 或 5% 降解的角叉菜胶，诱导损伤 30 至 44 天。在用降解角叉菜胶治疗的常规豚鼠中观察到盲肠或结肠溃疡、隐窝脓肿、粘膜变形、肠系膜淋巴结肿大、明显的盲肠或结肠淋巴增生以及其他病变。其他豚鼠用 2% 降解的角叉菜胶处理，但通过给予甲氧苄氨嘧啶和磺胺甲恶唑来清除肠杆菌科细菌，随后将其维持在隔离器中，几乎没有表现出任何病变。用 5% 降解角叉菜胶处理的无肠杆菌科动物的病变明显少于在常规降解角叉菜胶处理的动物中观察到的病变。细菌分离株的生物分型表明，肠杆菌科物种通常与病变相关，而不是单一生物型。据推测，细菌在穿透肠粘膜屏障后会刺激免疫反应。因此，用降解的角叉菜胶诱导的豚鼠炎症性肠病可能为研究人类炎症性肠病的免疫学方面提供有用的模型。
Experimentally induced ulcerative disease of the large intestine in guinea pigs was significantly mitigated by selective elimination of the aerobic gram-negative intestinal microflora. Lesions were induced with 2 or 5% degraded carrageenan administered in the drinking water for 30 to 44 days. Craterous cecal or colonic ulcers, crypt abscesses, mucosal distortion, mesenteric lymphadenopathy, marked cecal or colonic lymphoid hyperplasia, and other lesions were seen in conventional guinea pigs treated with degraded carrageenan. Other guinea pigs that were treated with 2% degraded carrageenan, but freed of Enterobacteriaceae species by the administration of trimethoprim with sulfamethoxazole, and subsequently maintained in isolators, exhibited virtually no lesions. Enterobacteriaceae-free animals that were treated with 5% degraded carrageenan had significantly fewer lesions than were observed in the conventional degraded carrageenan-treated animals. Biotyping of bacterial isolates indicated that the family of Enterobacteriaceae species generally, and not a single biotype, was associated with the lesions. The bacteria presumably stimulated an immunological response after penetration of the intestinal mucosal barrier; thus, inflammatory bowel disease in guinea pigs, induced with degraded carrageenan, may provide a useful model for investigating immunological aspects of human inflammatory bowel disease.