Double sex and mab-3 related transcription factor 1 regulates differentiation and proliferation in dairy goat male germline stem cells
Double sex and mab-3 related transcription factor 1 regulates differentiation and proliferation in dairy goat male germline stem cells
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双性和mab-3相关转录因子1调节奶山羊雄性种系干细胞的分化和增殖
DOI：
10.1002/jcp.26129
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Jinlian Hua
中科院分区：
文献类型：
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作者：
Yudong Wei;Shufang Cai;Fanglin Ma;Ying Zhang;Zhe Zhou;Shuanshuan Xu;Mengfei Zhang;Sha Peng;Jinlian Hua
双性和单抗- 3相关转录因子1 （Dmrt1）基因编码的蛋白含有双性/单抗- 3结构域，该结构域被认为是性别决定中最保守的结构之一。然而，其对奶山羊精原干细胞（ssc）精子发生的影响尚不清楚。本文首次强调了Dmrt1在家畜精子发生中的作用。在此，我们研究了Dmrt1在奶山羊睾丸中的表达模式。Dmrt1主要位于未分化的ssc中。此外，Dmrt1增强了mGSCs的分化和增殖。相反，减数分裂水平下调，因为Dmrt1决定ssc是否进行有丝分裂和精原细胞分化或减数分裂。在busulfan处理的小鼠睾丸中，Dmrt1修复生殖细胞损伤也被强调。我们的研究结果表明，Dmrt1通过两种方式维持mGSCs：促进SSCs的增殖和自我更新；并减少由生殖损伤引起的炎症反应。这些发现确定了Dmrt1在控制ssc种群稳定和损伤恢复中的核心作用。
The protein encoded by double sex and mab‐3 related transcription factor 1 (Dmrt1) gene contains a double sex/mab‐3 domain, which was considered as one of the most conservative structures in sex determination. However, its effect on spermatogenesis of dairy goat spermatogonial stem cells (SSCs) remains to be clarified. For the first time, the roles of Dmrt1 in spermatogenesis of livestock are highlighted. Here, we investigated the expression pattern of Dmrt1 in the testes of dairy goats. Dmrt1 primarily located in undifferentiated SSCs. Moreover, Dmrt1 enhanced differentiation and proliferation of mGSCs. On the contrary, the level of meiosis was down‐regulated, as Dmrt1 determines whether SSCs undergo mitosis and spermatogonial differentiation or meiosis. In the busulfan‐treated mice testes, Dmrt1 repair germ cell damage was emphasized as well. Our results exposed that Dmrt1 maintenance mGSCs in two ways: facilitating proliferation and self‐renewal of SSCs; and reducing the inflammatory response caused by reproductive injury. These findings identify a central role for Dmrt1 in controlling population stability and injury restoring of SSCs.