THEORY OF DNA MELTING CURVES
THEORY OF DNA MELTING CURVES
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DOI：
10.1002/bip.1977.360161209
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发表时间：
1977-01-01
期刊：
影响因子：
2.9
通讯作者：
FREIRE, JJ
中科院分区：
文献类型：
--
作者：
FIXMAN, M;FREIRE, JJ
计算具有N个碱基对的异质DNA熔化曲线的精确算法显然需要与n2成正比的计算机时间。将循环熵因子分解成i个指数函数的和给出了对于i的小值的循环熵因子的极其精确的近似，并且使得精确算法的计算机时间与i成正比。用与Frank-Kamenetskii近似相当的计算机时间，得到了螺旋或线圈拉伸的熔化曲线和长度的精确结果。后者对于螺旋含量的分数具有显著的准确性(误差为0.01-0.05)，但对于螺旋线或线圈长度的误差要大得多(典型误差为30%-100%)。
Exact algorithms for the calculation of melting curves of heterogeneous DNA with N base pairs apparently require computer time proportional to N2. A decomposition of the loop entropy factor into a sum of I exponential functions gives an extremely accurate approximation to the loop entropy factor for small values of I and makes the computer time for the exact algorithms proportional to I .cntdot. N. Exact results for melting curves and lengths of helix or coil stretches are obtained with computer time comparable to that required for the Frank-Kamenetskii approximation. The remarkable accuracy of the latter for the fraction of helical content (errors of 0.01-0.05) is confirmed, but appreciably larger errors are found for the lengths of helix or coil stretches (typical errors of 30-100%).