Transcription activation of the tyrosine aminotransferase gene by glucocorticoids and cAMP in primary hepatocytes.
Transcription activation of the tyrosine aminotransferase gene by glucocorticoids and cAMP in primary hepatocytes.
复制标题
原代肝细胞中糖皮质激素和 cAMP 对酪氨酸转氨酶基因的转录激活。
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
G. Schütz
中科院分区：
文献类型：
--
作者：
E. Schmid;W. Schmid;Michael Jantzen;D. Mayer;B. Jastorff;G. Schütz
分析了大鼠原代肝细胞中酪氨酸转氨酶（TAT）基因的表达。TAT基因在原代培养细胞中保持活性，其水平与肝细胞相似。糖皮质激素和cAMP均可诱导表达，糖皮质激素导致TAT mRNA水平升高8-10倍，cAMP升高20-30倍。在TAT mRNA的升高之前，该基因的相对转录率升高。令人惊讶的是，随着培养年龄的增长而稳步下降的白蛋白基因的转录，也可以被原代肝细胞中的糖皮质激素强烈刺激。cAMP拮抗剂作为cAMP依赖性蛋白激酶的竞争性抑制剂，阻止了cAMP对酪氨酸转氨酶基因转录的诱导，这表明cAMP对酪氨酸转氨酶基因表达的影响是由cAMP依赖性蛋白激酶介导的。cAMP拮抗剂不会干扰糖皮质激素的诱导，这表明cAMP依赖性蛋白激酶对糖皮质激素受体的磷酸化不是其功能所必需的。因此，我们得出结论，这两种诱导剂通过独立的机制影响转录。
The expression of the tyrosine aminotransferase (TAT) gene of the rat was analyzed in primary hepatocytes. The TAT gene remains active in primary cultured cells at a level similar to that in liver cells. Expression can be induced by glucocorticoids and cAMP, glucocorticoids lead to a 8-10-fold increase in TAT mRNA level, cAMP to a 20-30-fold increase. The elevation of the TAT mRNA is preceeded by a rise in the relative rate of transcription of the gene. Surprisingly transcription of the albumin gene, which steadily declines with the age of the culture, can also strongly be stimulated by glucocorticoids in primary hepatocytes. cAMP antagonists, which act as competitive inhibitors of the cAMP-dependent protein kinase, prevent induction of transcription of the tyrosine aminotransferase gene by cAMP suggesting that the effect of cAMP on expression of the tyrosine aminotransferase gene is mediated by a cAMP-dependent protein kinase. The cAMP antagonist does not interfere with induction by glucocorticoids which suggests that phosphorylation of the glucocorticoid receptor by the cAMP-dependent protein kinase is not required for its function. We thus conclude that the two inducers affect transcription by independent mechanisms.
与纯化的大鼠肝糖皮质激素受体相关的蛋白激酶活性。
DOI：
10.1073/pnas.82.12.4003
发表时间：
1985
影响因子：
11.1
作者：
Miller-Diener,A;Schmidt,TJ;Litwack,G
通讯作者：
Litwack,G
小鼠 7 号染色体上白化基因座附近的缺失会影响酪氨酸转氨酶 mRNA 的水平。
DOI：
10.1073/pnas.82.9.2866
发表时间：
1985
影响因子：
11.1
作者：
Schmid,W;Müller,G;Schütz,G;Gluecksohn-Waelsch,S
通讯作者：
Gluecksohn-Waelsch,S
完整 L 细胞直接证明糖皮质激素受体磷酸化。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Housley,PR;Pratt,WB
通讯作者：
Pratt,WB