Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni
Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni
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DOI：
10.1007/978-1-4939-6536-6_3
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Brondsted, Lone
中科院分区：
文献类型：
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作者：
Gencay, Yilmaz Emre;Birk, Tina;Brondsted, Lone
在这里，我们描述了从预计含有大量噬菌体（例如鸡粪便）的样品中分离、纯化和繁殖空肠弯曲杆菌噬菌体的方法。总体步骤是（1）从样品材料中释放噬菌体； (2)利用斑点测定法观察空肠弯曲菌菌苔上的噬菌斑形成单位； (3)单个斑块的分离； (4)连续纯化程序； (5)从平板裂解物中繁殖纯化的噬菌体以制备母液。
Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.