Clock gene expression in the submandibular glands
Clock gene expression in the submandibular glands
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DOI：
10.1177/154405910508401219
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发表时间：
2005-12-01
影响因子：
7.6
通讯作者：
Kato, Y
中科院分区：
文献类型：
--
作者：
Furukawa, M;Kawamoto, T;Kato, Y
介导分子昼夜节律的时钟基因在视交叉上核和各种外周组织中以昼夜节律方式表达。为了建立唾液腺昼夜节律调节的分子基础，我们检查了时钟相关基因和唾液腺特征基因的表达谱。时钟相关基因 - 包括 Per1、Per2、Cry1、Bmal1、Dec1、Dec2、Dbp 和 Reverb α - 在 12:12 小时明暗条件下在颌下腺中显示出强大的昼夜节律表达节律。此外，在淀粉酶 1 mRNA 水平中观察到强大的昼夜节律，而检查的其他唾液腺特征基因的表达没有节律。 Clock突变导致Per2、Bmal1、Dec1、Dec2和Dbp的mRNA水平增加或降低，并且在Cry1-/-背景下，Cry2破坏也增加或降低了这些时钟相关基因和淀粉酶1基因的mRNA水平。这些发现表明时钟和哭声依赖性分子钟系统在唾液腺中活跃。
Clock genes, which mediate molecular circadian rhythms, are expressed in a circadian fashion in the suprachiasmatic nucleus and in various peripheral tissues. To establish a molecular basis for circadian regulation in the salivary glands, we examined expression profiles of clock- related genes and salivary gland- characteristic genes. Clock- related genes - including Per1, Per2, Cry1, Bmal1, Dec1, Dec2, Dbp, and Rev- erb alpha- showed robust circadian expression rhythms in the submandibular glands in 12: 12- hour light- dark conditions. In addition, a robust circadian rhythm was observed in amylase 1 mRNA levels, whereas the expression of other salivary- glandcharacteristic genes examined was not rhythmic. The Clock mutation resulted in increased or decreased mRNA levels of Per2, Bmal1, Dec1, Dec2, and Dbp, and in Cry1-/- background, Cry2 disruption also increased or decreased mRNA levels of these clock- related genes and the amylase 1 gene. These findings indicate that the Clock- and Cry- dependent molecular clock system is active in the salivary glands.