Fluctuation of the SP/non-SP phenotype in the C6 glioma cell line
Fluctuation of the SP/non-SP phenotype in the C6 glioma cell line
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DOI：
10.1016/j.febslet.2007.02.071
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发表时间：
2007-04-03
期刊：
影响因子：
3.5
通讯作者：
Wion, Didier
中科院分区：
文献类型：
--
作者：
Platet, Nadine;Mayol, Jean-Francois;Wion, Didier
使用C6胶质瘤细胞作为范例，我们发现（i）C6细胞的克隆形成性比SP细胞的百分比高几个数量级;（ii）非SP细胞能够产生SP细胞，并且相反地SP细胞产生非SP细胞;（iii）非SP分选的细胞表现为致瘤细胞。因此，在含血清培养基中培养的C6细胞中，SP细胞可以由非SP细胞产生。这种动态平衡解释了C6细胞中SP表型随细胞传代的维持，并证明了致瘤性非SP细胞的存在。(c)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Using the C6 glioma cell as a paradigm, we found that (i) the clonogenicity of C6 cells is several orders of magnitude higher than the percentage of SP cells; (ii) non-SP cells are able to generate SP cells, and conversely SP cells generate non-SP cells; (iii) non-SP sorted cells behave as tumorigenic cells. Hence, in C6 cells cultured in serum-containing medium, SP cells can be generated from non-SP cells. This dynamic equilibrium explains in C6 cells the maintenance of the SP phenotype with cell passaging and demonstrates the existence of tumorigenic non-SP cells. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.