Interval mapping of quantitative trait loci for resistance to late blight [Phytophthora infestans (Mont.) de Bary], height and maturity in a tetraploid population of potato (Solanum tuberosum subsp tuberosum)
Interval mapping of quantitative trait loci for resistance to late blight [Phytophthora infestans (Mont.) de Bary], height and maturity in a tetraploid population of potato (Solanum tuberosum subsp tuberosum)
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DOI：
10.1534/genetics.104.030056
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发表时间：
2004-10-01
期刊：
影响因子：
3.3
通讯作者：
Waugh, R
中科院分区：
文献类型：
--
作者：
Bradshaw, JE;Pande, B;Waugh, R
对感病亲本12601ab1与抗病品种Stirling杂交的227个无性系组成的四倍体马铃薯全同胞家系进行了抗晚疫病、株高和成熟度的QTL区间定位。38个AFLP引物组合提供了585个信息性标记，23个SSR标记对连锁群的识别是有用的。在Stirling的LGV上检测到一个与早熟、矮秆和感病相关的单QTL等位基因，可解释54.7%、26.5%、26.3%和17.5%的成熟度、株高、块茎枯病和叶枯病的遗传变异。当分析叶片和块茎枯病对成熟度的回归残差时，LGV没有显著的QTL效应，但在Stirling的LGIV上发现了一个双重QTL等位基因，可以解释加性模型下叶片和块茎枯病变异的30.7%和13.6%。抗性的部分显性解释了更多的变异，对叶枯病的解释高达37.2%。Stirling的一个抗病主效基因也被定位到LGXI。
Interval mapping of quantitative trait loci (QTL) for resistance to late blight, height, and maturity was performed on a tetraploid full-sib family of potato comprising 227 clones from a cross between a susceptible parent, 12601ab1, and a resistant cultivar, Stirling, which were of similar height and main crop maturity. Thirty-eight AFLP primer combinations provided 585 informative markers, and 23 SSRs proved useful for identifying linkage groups (LGs). A simplex QTL allele was found on LGV of Stirling close to marker STM3179, which was associated with early maturity, short plants, and susceptibility to blight and explained 54.7, 26.5, 26.3, and 17.5% of the variation for maturity, height, tuber blight, and foliage blight. When the residuals from the regressions of foliage and tuber blight on maturity were analyzed, there was no significant effect of a QTL on LGV, but a duplex QTL allele for resistance was found on LGIV of Stirling, which explained 30.7 and 13.6% of the variation for foliage and tuber blight on an additive model. Partial dominance for resistance explained even more of the variation, up to 37.2% for foliage blight. A major gene for blight resistance in Stirling was also mapped to LGXI.