OTX1 is a novel regulator of proliferation, migration, invasion and apoptosis in lung adenocarcinoma
OTX1 is a novel regulator of proliferation, migration, invasion and apoptosis in lung adenocarcinoma
复制标题
OTX1是肺腺癌增殖、迁移、侵袭和凋亡的新型调节因子
DOI：
10.26355/eurrev_202009_23035
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发表时间：
2020-01-01
影响因子：
3.3
通讯作者：
Zheng, S-Y
中科院分区：
文献类型：
--
作者：
Yang, C-Y;Wang, L.;Zheng, S-Y
目的：已发现正牙本质同源盒1（OTX 1）与多种人类肿瘤的发生密切相关。然而，OTX 1在非小细胞肺癌（NSCLC）中的功能和潜在的分子机制尚不清楚。本研究旨在探讨下调OTX 1基因表达对人NSCLC细胞系增殖、迁移、侵袭、细胞周期和凋亡的影响。患者和方法：用对照小干扰RNA（siNC）或实验siRNA（siOTX 1）转染培养的NCI-H292和XWLC细胞。使用定量实时PCR（RT-qPCR）测定来检测mRNA水平。使用细胞计数试剂盒-8（CCK-8）和真实的时间细胞分析仪（RTCA）测定细胞活性。RTCA和transwell小室用于评估细胞迁移和侵袭。结果：与对照组相比，实验组细胞活性明显降低（*** p <0.01），细胞周期进程和凋亡率明显降低（*** p <0.01），细胞凋亡率明显降低（***p <0.01），细胞凋亡率明显降低（***p <0.01），细胞凋亡率明显降低（***p <0.01）。
OBJECTIVE: Orthodenticle Homeobox 1 (OTX1) has been found to be closely related to the development of several human tumours. However, the function and underlying molecular mechanisms of OTX1 in non-small cell lung cancer (NSCLC) are unclear. This research was performed to investigate the effects of downregulating OTX1 gene expression on the proliferation, migration, invasion, cell cycle and apoptosis of human NSCLC cell lines.PATIENTS AND METHODS: Cultured NCI-H292 and XWLC cells were transfected with control small interfering RNA (siNC) or experimental siRNA (siOTX1). The mRNA levels were detected using a quantitative real-time PCR (RT-qPCR) assay. A Cell Counting Kit-8 (CCK-8) and a Real Time Cell Analyzer (RTCA) were used to determine cell activity. The RTCA and transwell chambers were used to assess cell migration and invasion. In addition, cell cycle progression and apoptosis were measured using flow cytometry, and the expression levels of key signalling pathway proteins were examined by Western blotting.RESULTS: The results revealed that compared with the control group, the experimental group exhibited significantly decreased cell activity (***p