A Ratiometric Photoacoustic Probe with a Reversible Response to Hydrogen Sulfide and Hydroxyl Radicals for Dynamic Imaging of Liver Inflammation
A Ratiometric Photoacoustic Probe with a Reversible Response to Hydrogen Sulfide and Hydroxyl Radicals for Dynamic Imaging of Liver Inflammation
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对硫化氢和羟基自由基具有可逆响应的比例光声探针，用于肝脏炎症的动态成像
DOI：
10.1002/anie.202209248
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Ye Deju
中科院分区：
文献类型：
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作者：
Wu Luyan;Zeng Wenhui;Ishigaki Yusuke;Zhang Junya;Bai He;Harimoto Takashi;Suzuki Takanori;Ye Deju
可逆成像探针允许羟基自由基（OH）和硫化氢（H2S）之间氧化还原循环的动态可视化，对于探测体内氧化还原失衡相关的病理过程至关重要。在此，我们报告了一种可逆的比率光声（PA）成像纳米探针（1-PAIN），用于体内2，3，4-二氢氧化铝/H2S氧化还原循环的真实的-时间成像。1-PAIN在690和825 nm之间显示低PA比率 （PA 690/PA 825），在被NH 4 OH氧化后显著增加0.55倍，并在H2S还原后转换回最初的低PA 690/PA 825值。1-PAIN可以动态报告脂多糖（LPS）诱导的肝脏炎症过程中小鼠肝脏中的β-羟色胺产生，并在N-乙酰半胱氨酸（NAC）诱导的抗炎过程中可视化肝脏H2S的产生。1-PAIN可以作为探测生物体内氧化还原状态的有用工具，有利于氧化还原失衡相关疾病的研究。
Reversible imaging probes that allow for the dynamic visualization of the redox cycle between hydroxyl radical (⋅OH) and hydrogen sulfide (H2S) are vital to probe the redox imbalance‐involved pathological process in vivo. Herein, we report a reversible ratiometric photoacoustic (PA) imaging nanoprobe (1‐PAIN) for the real‐time imaging of ⋅OH/H2S redox cycle in vivo.1‐PAIN displays a low PA ratio between 690 and 825 nm (PA690/PA825), which significantly increases by ≈5‐fold upon oxidation by ⋅OH, and is switched back to the initially low PA690/PA825value upon reduction by H2S.1‐PAIN could dynamically report on the hepatic ⋅OH production in mice during the lipopolysaccharide (LPS)‐induced liver inflammation process, and visualize hepatic H2S generation during theN‐acetyl cysteine (NAC)‐induced anti‐inflammation process.1‐PAIN can act as a useful tool to probe the redox state in living biology, beneficial for the study of redox imbalance‐related diseases.