ANALYSIS OF THE BRASSICA-OLERACEA GENOME BY THE GENERATION OF B CAMPESTRIS-OLERACEA CHROMOSOME ADDITION LINES - CHARACTERIZATION BY ISOZYMES AND RDNA GENES
ANALYSIS OF THE BRASSICA-OLERACEA GENOME BY THE GENERATION OF B CAMPESTRIS-OLERACEA CHROMOSOME ADDITION LINES - CHARACTERIZATION BY ISOZYMES AND RDNA GENES
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DOI：
10.1007/bf00247554
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发表时间：
1987-01-01
影响因子：
5.4
通讯作者：
DOUCHES, D
中科院分区：
文献类型：
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作者：
QUIROS, CF;OCHOA, O;DOUCHES, D
本研究旨在建立芸苔属染色体附加系，揭示基因组特异性标记。这些砧木将用于研究甘蓝、油菜及其衍生的双二倍体种油菜的基因组进化。油菜-油菜单体染色体和二染色体附加植株是由天然的两二倍体油菜与二倍体亲本种油菜进行杂交和回交而产生的。获得的倍半二倍体和超倍体植株的花粉育力为63% ~ 88%，单体和二体植株的平均花粉育力分别为94%和91%。利用基因组特异性标记对附加品系进行遗传鉴定。6PGD， LAP同工酶。发现PGI和PGM以及rDNA Eco RI限制性内切片段具有所需的基因组特异性。在油菜芽孢杆菌和甘蓝芽孢杆菌中发现了这些标记的重复位点，支持了这些二倍体物种实际上是次生多倍体的假设。利用这些标记对8个单体和8个二体添加植株进行了鉴定和鉴定。另有51株植物由于缺乏额外的标记而未被鉴定。发现rDNA基因分布在不止一条染色体上，其限制性位点不同。部分标记的基因组间重组频率在6% ~ 20%之间，揭示了基因组间基因转移的可行性。
This study aimed at generating chromosome addition lines and disclosing genome specific markers in Brassica. These stocks will be used to study genome evolution in Brassica oleracea L., B. campestris L. and the derived amphidiploid species B. napus L. B. campestris-oleracea monosomic and disomic chromosome addition plants were generated by crossing and backcrossing the natural amphidiploid B. napus to the diploid parental species B. campestris. The pollen viability of the derived sesquidiploid and hyperploid ranged from 63% to 88% while the monosomic and disomic addition plants had an average pollen fertility of 94% and 91%, respectively. The addition lines were genetically characterized by genome specific markers. The isozymes for 6PGD, LAP. PGI and PGM, and rDNA Eco RI restriction fragments were found to possess the desired genome specificity. Duplicated loci for several of these markers were observed in B. campestris and B. oleracea, supporting the hypothesis that these diploid species are actually secondary polyploids. A total of eight monosomic and eight disomic addition plants were identified and characterized on the basis of these markers. Another 51 plants remained uncharacterized due to the lack of additional markers. rDNA genes were found to be distributed in more than one chromosome, differing in its restriction sites. Intergenomic recombination for some of the markers was detected at frequencies between 6% and 20%, revealing the feasibility of intergenomic gene transfer.