Peroxisomes in human and mouse testis:: Differential expression of peroxisomal proteins in germ cells and distinct somatic cell types of the testis
Peroxisomes in human and mouse testis:: Differential expression of peroxisomal proteins in germ cells and distinct somatic cell types of the testis
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DOI：
10.1095/biolreprod.107.061242
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发表时间：
2007-12-01
影响因子：
3.6
通讯作者：
Baumgart-Vogt, Eveline
中科院分区：
文献类型：
--
作者：
Nenicu, Anca;Luers, Georg H.;Baumgart-Vogt, Eveline
过氧化物酶体代谢对于睾丸正常功能至关重要，过氧化物酶体功能障碍引起的严重精子发生缺陷强调了过氧化物酶体代谢对于睾丸正常功能的至关重要性。然而，关于该细胞器在睾丸不同细胞类型中的作用和酶组成的信息很少。在本研究中，我们采用多种形态学和生化技术，在鼠体细胞（支持细胞、管周肌样细胞和间质细胞）的原代培养物和 GFP-PTS1 转基因小鼠中，对人和小鼠睾丸的过氧化物酶体区室进行了表征。以前，过氧化物酶体被认为在精子发生的后期不存在。然而，我们通过检测不同的过氧化物酶体标记蛋白获得的结果表明，除成熟精子外，睾丸中大多数细胞类型中都存在这些细胞器。此外，我们证明了人和小鼠睾丸各种细胞类型中过氧化物酶体蛋白含量的强烈异质性，并显示出精子细胞中这些细胞器的结构、丰度和定位的显着差异，具体取决于它们的成熟程度。在支持细胞中发现了过氧化物酶体脂质转运蛋白（ABCD1 和 ABCD3）以及 ACOX2（胆固醇侧链氧化的 β-氧化途径 2 的关键调节酶）的最高和选择性富集，而 Leydig 细胞中过氧化氢酶和 ABCD2 富集。我们的结果表明过氧化物酶体在睾丸中具有细胞类型特异性代谢功能，并指出过氧化物酶体在精子发生和支持细胞的脂质代谢中发挥重要作用。
The vital importance of peroxisomal metabolism for regular function of the testis is stressed by the severe spermatogenesis defects induced by peroxisomal dysfunction. However, only sparse information is available on the role and enzyme composition of this organelle in distinct cell types of the testis. In the present study, we characterized the peroxisomal compartment in human and mouse testis in primary cultures of murine somatic cells (Sertoli, peritubular myoid, and Leydig cells) and in GFP-PTS1 transgenic mice with a variety of morphological and biochemical techniques. Formerly, peroxisomes were thought to be absent in late stages of spermatogenesis. However, our results obtained by detection of different peroxisomal marker proteins show the presence of these organelles in most cell types in the testis, except for mature spermatozoa. Furthermore, we demonstrate a strong heterogeneity of peroxisomal protein content in various cell types of the human and mouse testis and show marked differences in structure, abundance, and localization of these organelles in spermatids, depending on their maturation. Highest and selective enrichment of the peroxisomal lipid transporters (ABCD1 and ABCD3) as well as ACOX2, the key regulatory enzyme of the beta-oxidation pathway 2 for side chain oxidation of cholesterol, were found in Sertoli cells, whereas Leydig cells were enriched in catalase and ABCD2. Our results suggest a cell type-specific metabolic function of peroxisomes in the testis and point to an important role for peroxisomes in spermiogenesis and in the lipid metabolism of Sertoli cells.