Regulation of the chemosensory control of breathing by Kolliker-Fuse neurons
Regulation of the chemosensory control of breathing by Kolliker-Fuse neurons
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DOI：
10.1152/ajpregu.00024.2014
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发表时间：
2014-07-01
影响因子：
2.8
通讯作者：
Moreira, Thiago S.
中科院分区：
文献类型：
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作者：
Damasceno, Roselia S.;Takakura, Ana C.;Moreira, Thiago S.
Kolliker-Bronze区（KF）和臂旁外侧核（LPBN）与维持心肺控制有关.在这里，我们评估了KF区域和LPBN在未麻醉大鼠化学感受器激活引起的心肺反应的参与。使用在KF区域或LPBN中植入双侧不锈钢引导套管的雄性Wistar大鼠（280-330 g; n = 5-9/组）。在KF区域注射蝇蕈醇（100和200 pmol/100 nl）可降低静息通气量（1，140 +/- 68和978 +/- 100 vs.盐水：1，436 +/- 155 ml）。kg（-1）。min（-1）），而不改变平均动脉压（MAP）和心率（HR）。在KF中双侧注射GABA-A拮抗剂荷包牡丹碱（1 nmol/100 nl）阻断了对通气的抑制作用（1，418 +/- 138 vs.蝇蕈醇：978 +/- 100 ml）。kg（-1）。min（-1））。KF中的蝇蕈醇注射减少了缺氧（8% O-2）引起的通气增加（1，827 +/- 61 vs.盐水：3，179 +/- 325 ml）。kg（-1）。min（-1））或高碳酸血症（7% CO2）（1，488 +/- 277 vs.盐水：3，539 +/- 374 ml . kg（-1）。min（-1））。双侧注射荷包牡丹碱在KF阻断了减少通风产生的蝇蕈醇在KF外周或中枢化学反射激活。在LPBN中双侧注射蝇蕈醇并不改变静息通气或由缺氧或高碳酸血症引起的通气增加。本研究的结果表明，KF区域，而不是LPBN，有机制来控制通气在休息，缺氧，或高碳酸条件下，在未麻醉大鼠。
The Kolliker- Fuse region (KF) and the lateral parabrachial nucleus (LPBN) have been implicated in the maintenance of cardiorespiratory control. Here, we evaluated the involvement of the KF region and the LPBN in cardiorespiratory responses elicited by chemoreceptor activation in unanesthetized rats. Male Wistar rats (280-330 g; n = 5-9/group) with bilateral stainless-steel guide cannulas implanted in the KF region or the LPBN were used. Injection of muscimol (100 and 200 pmol/100 nl) in the KF region decreased resting ventilation (1,140 +/- 68 and 978 +/- 100 vs. saline: 1,436 +/- 155 ml . kg(-1) . min(-1)), without changing mean arterial pressure (MAP) and heart rate (HR). Bilateral injection of the GABA-A antagonist bicuculline (1 nmol/100 nl) in the KF blocked the inhibitory effect on ventilation (1,418 +/- 138 vs. muscimol: 978 +/- 100 ml . kg(-1) . min(-1)) elicited by muscimol. Muscimol injection in the KF reduced the increase in ventilation produced by hypoxia (8% O-2) (1,827 +/- 61 vs. saline: 3,179 +/- 325 ml . kg(-1) . min(-1)) or hypercapnia (7% CO2) (1,488 +/- 277 vs. saline: 3,539 +/- 374 ml . kg(-1) . min(-1)) in unanesthetized rats. Bilateral injection of bicuculline in the KF blocked the decrease in ventilation produced by muscimol in the KF during peripheral or central chemoreflex activation. Bilateral injection of muscimol in the LPBN did not change resting ventilation or the increase in ventilation elicited by hypoxia or hypercapnia. The results of the present study suggest that the KF region, but not the LPBN, has mechanisms to control ventilation in resting, hypoxic, or hypercapnic conditions in unanesthetized rats.