RNA polymerase can track a DNA groove during promoter search
RNA polymerase can track a DNA groove during promoter search
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DOI：
10.1073/pnas.0406441101
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发表时间：
2004-10-12
影响因子：
11.1
通讯作者：
Shimamoto, N
中科院分区：
文献类型：
--
作者：
Sakata-Sogawa, K;Shimamoto, N
许多蛋白质选择特殊的DNA序列来形成功能复合物。在一种可能的机制中，蛋白质分子将通过跟踪没有完全解离的凹槽来扫描DNA序列。将单个DNA分子拖过携带固定大肠杆菌RNA聚合酶全酶的表面，我们检测到单个DNA分子的旋转，提供了DNA结合蛋白可以跟踪DNA凹槽的直接证据。这些结果证实了我们之前关于RNA聚合酶沿着固定的、延伸的DNA纵向运动的观察，而且，这意味着凹槽跟踪有助于DNA序列的扫描。
Many proteins select special DNA sequences to form functional complexes. In one possible mechanism, protein molecules would scan DNA sequences by tracking a groove without complete dissociation. Upon dragging single molecules of DNA over a surface carrying fixed Escherichia coli RNA polymerase holoenzyme, we detected rotation of individual DNA molecules, providing direct evidence that a DNA-binding protein can track a DNA groove. These results confirm our previous observations of longitudinal movement of RNA polymerase along fixed, extended DNA and, moreover, imply that groove tracking facilitates scanning of DNA sequences.