COOPERATIVITY IN ATP HYDROLYSIS BY GROEL IS INCREASED BY GROES
COOPERATIVITY IN ATP HYDROLYSIS BY GROEL IS INCREASED BY GROES
复制标题
DOI：
10.1016/0014-5793(91)80878-7
复制
发表时间：
1991-11-04
期刊：
影响因子：
3.5
通讯作者：
FERSHT, AR
中科院分区：
文献类型：
--
作者：
GRAY, TE;FERSHT, AR
更详细地研究了分子伴侣GroEL对ATP的水解动力学以及GroES对这种水解的抑制作用。结果表明，GroEL与ATP是协同作用的，其Hill系数为1.86(+/-0.13)。在GROE存在下，协同度增加，希尔系数为3.01(+/-0.18)。观察到的协同性并不是由于GroEL齐聚物解离成更小的单元，而是更可能涉及GroEL齐聚物内部的结构变化。
The kinetics of ATP hydrolysis by the 'molecular chaperone' GroEL and the inhibition of this hydrolysis by GroES have been studied in more detail. It is shown that the hydrolysis of ATP by GroEL is cooperative with respect to ATP with a Hill coefficient of 1.86 (+/- 0.13). In the presence of GroES, there is an increase in the degree of cooperativity with a Hill coefficient of 3.01 (+/- 0.18). The observed cooperativity is not due to dissociation of the GroEL oligomer into smaller units but more probably involves structural changes within the GroEL oligomer.