Determination of indican, isatin, indirubin and indigotin in Isatis indigotica by liquid chromatography/electrospray ionization tandem mass spectrometry
Determination of indican, isatin, indirubin and indigotin in Isatis indigotica by liquid chromatography/electrospray ionization tandem mass spectrometry
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DOI：
10.1002/rcm.2954
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发表时间：
2007-01-01
影响因子：
2
通讯作者：
Koh, Hwee Ling
中科院分区：
文献类型：
--
作者：
Zou, Peng;Koh, Hwee Ling
菘蓝的根和叶分别命名为“板蓝根”和“大青叶”，广泛用于治疗流感、病毒性肺炎、腮腺炎、咽炎和肝炎。据报道，在靛蓝根和叶中检测到的吲哚酚衍生物具有生物活性。本研究建立了液相色谱/电喷雾串联质谱（LC/ESI-MS/MS）方法来测定靛蓝根和叶中的靛蓝、靛红、靛玉红和靛蓝。该方法的线性度、精密度和准确度已得到验证。使用多反应监测 (MRM)，检测限 (LOD) 确定为：靛蓝 0.004 ng、靛红 0.01 ng、靛玉红 0.01 ng 和靛蓝 0.03 ng，定量限 (LOQ) 为靛蓝 0.015 ng、靛红 0.04 ng、靛蓝 0.04 ng。靛玉红和 0.1 纳克靛蓝。与之前报道的方法相比，该方法更加快速、选择性和灵敏。这是 LC/MS/MS 测定靛蓝、靛红、靛玉红和靛蓝的第一份报告。版权所有 (c) 2007 John Wiley & Sons, Ltd.
The roots and leaves of Isatis indigotica, named 'Ban-Lan-Gen' and 'Da-Qing-Ye', respectively, are widely used for the treatment of influenza, viral pneumonia, mumps, pharyngitis, and hepatitis. The indoxyl derivatives detected in the roots and leaves of I. indigotica have been reported to be biologically active. In the present study, a liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method was developed to determine indican, isatin, indirubin and indigotin in the roots and leaves of L indigotica. The method has been validated for linearity, precision and accuracy. Using multiple reaction monitoring (MRM), the limits of detection (LODs) were determined as 0.004 ng for indican, 0.01 ng for isatin, 0.01 ng for indrubin and 0.03 ng for indigotin, while the limits of quantitation (LOQs) were 0.015 ng for indican, 0.04 ng for isatin, 0.04 ng for indirubin and 0.1 ng for indigotin. Compared with previously reported methods, the current method is more rapid, selective and sensitive. This is the first report of the LC/MS/MS determination of indican, isatin, indirubin and indigotin. Copyright (c) 2007 John Wiley & Sons, Ltd.