Screening of transgenic proteins expressed in transgenic food crops for the presence of short amino acid sequences identical to potential, IgE - binding linear epitopes of allergens.
Screening of transgenic proteins expressed in transgenic food crops for the presence of short amino acid sequences identical to potential, IgE - binding linear epitopes of allergens.
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DOI：
10.1186/1472-6807-2-8
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发表时间：
2002-12-12
影响因子：
--
通讯作者：
Peijnenburg, Ad A C M
中科院分区：
文献类型：
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作者：
Kleter, Gijs A;Peijnenburg, Ad A C M
背景：转基因粮食作物表达的转基因蛋白在上市前评估其潜在的致敏特性，其中包括鉴定转基因蛋白和致敏蛋白中存在的短相同氨基酸序列。提出了一种策略，即进一步筛选最小长度为6个氨基酸的序列比较的阳性结果，以确定潜在的线性IgE表位的存在。这种双轨方法包括使用关于IgE表位的文献数据和抗原性预测算法。结果：已经对33个转基因蛋白质进行了筛选，以确定至少6个与过敏性蛋白质共有的相邻氨基酸的身份。22个转基因蛋白显示出6个或7个相邻氨基酸长度的阳性结果。只有少数转基因蛋白(番木瓜环斑病毒外壳蛋白、乙酰乳酸合成酶GH50和草甘膦氧化还原酶)和致敏蛋白具有相同的长度，可以被鉴定为潜在的线性表位的一部分。大多数相同的伸展很可能是假阳性。如本研究所示，通过与文献中描述的线性IgE结合表位进行比较，可以进一步筛选相同的延伸片段的相关性。在没有关于表位的文献数据的情况下，通过计算机进行抗原性预测来选择潜在的抗体结合部位，这些抗体结合部位将需要通过血清结合试验来验证IgE结合。最后，这种方法的积极结果需要进一步临床测试潜在的过敏性。
BACKGROUND: Transgenic proteins expressed by genetically modified food crops are evaluated for their potential allergenic properties prior to marketing, among others by identification of short identical amino acid sequences that occur both in the transgenic protein and allergenic proteins. A strategy is proposed, in which the positive outcomes of the sequence comparison with a minimal length of six amino acids are further screened for the presence of potential linear IgE-epitopes. This double track approach involves the use of literature data on IgE-epitopes and an antigenicity prediction algorithm.RESULTS: Thirty-three transgenic proteins have been screened for identities of at least six contiguous amino acids shared with allergenic proteins. Twenty-two transgenic proteins showed positive results of six- or seven-contiguous amino acids length. Only a limited number of identical stretches shared by transgenic proteins (papaya ringspot virus coat protein, acetolactate synthase GH50, and glyphosate oxidoreductase) and allergenic proteins could be identified as (part of) potential linear epitopes.CONCLUSION: Many transgenic proteins have identical stretches of six or seven amino acids in common with allergenic proteins. Most identical stretches are likely to be false positives. As shown in this study, identical stretches can be further screened for relevance by comparison with linear IgE-binding epitopes described in literature. In the absence of literature data on epitopes, antigenicity prediction by computer aids to select potential antibody binding sites that will need verification of IgE binding by sera binding tests. Finally, the positive outcomes of this approach warrant further clinical testing for potential allergenicity.