Locomotor activity level monitoring using the Drosophila Activity Monitoring (DAM) System.
Locomotor activity level monitoring using the Drosophila Activity Monitoring (DAM) System.
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DOI：
10.1101/pdb.prot5518
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发表时间：
2010-11-01
影响因子：
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通讯作者：
Allada, Ravi
中科院分区：
文献类型：
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作者：
Pfeiffenberger, Cory;Lear, Bridget C;Allada, Ravi
成年人的行为分析已被用于确定昼夜节律基因在果蝇中取得了巨大的成功。特别是，自发活动测定可以在小种群甚至个体苍蝇中识别几天内改变的行为模式。商业上可获得的高效自动化系统允许从大量个体中连续收集数据，并且分析工具使得可以从每个实验中快速分析昼夜节律行为的多个方面。这些功能使得运动活性测定可用于高通量分析，从而快速发现许多果蝇昼夜节律基因并对其进行功能表征。这里描述的运动测定可以同时评估昼夜节律和睡眠行为，并且可以使用几种方法来分析从这样的测定产生的数据。本方案详细说明了TriKinetics的果蝇活动监测（DAM）系统的使用。简单地说，该系统记录来自保持在放置在活动监测器中的密封管中的个体蝇的活动。一束红外线光束穿过每个管子的中点，每次苍蝇穿过光束时，都会测量到一个“活动事件”。在每个连续采样间隔的过程中检测到的事件被求和，并在每个果蝇的实验过程中记录。这里描述的一般方法可以应用于广泛的行为活动实验，包括睡眠剥夺分析和活动减退和活动过度的一般研究。
Adult behavioral assays have been used with great success in Drosophila melanogaster to identify circadian rhythm genes. In particular, the locomotor activity assay can identify altered behavior patterns over the course of several days in small populations, or even individual flies. Commercially available, highly efficient automated systems allow for continuous data collection from large numbers of individuals, and analytical tools make it possible to quickly analyze multiple aspects of circadian behavior from each experiment. These features make the locomotor activity assay useful for high-throughput analyses, leading to the rapid discovery and functional characterization of many Drosophila circadian rhythm genes. The locomotor assay described here can simultaneously assess both circadian and sleep behavior, and several methods can be used to analyze the data generated from such assays. This protocol details the use of the Drosophila Activity Monitoring (DAM) System from TriKinetics. Briefly, the system records activity from individual flies maintained in sealed tubes placed in activity monitors. An infrared beam directed through the midpoint of each tube measures an "activity event" each time a fly crosses the beam. Events detected over the course of each consecutive sampling interval are summed and recorded over the course of the experiment for each fly. The general approaches described here can be applied to a wide range of behavioral activity experiments, including sleep deprivation analyses and general studies of hypoactivity and hyperactivity.