Cortex, a Drosophila gene required to complete oocyte meiosis, is a member of the Cdc20/fizzy protein family
Cortex, a Drosophila gene required to complete oocyte meiosis, is a member of the Cdc20/fizzy protein family
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DOI：
10.1002/gene.1017
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发表时间：
2001-03-01
期刊：
影响因子：
1.5
通讯作者：
Strickland, S
中科院分区：
文献类型：
--
作者：
Chu, T;Henrion, G;Strickland, S
皮质和grauzone的突变导致果蝇雌性减数分裂异常停滞。通过雄性重组作图法将皮层定位到26 F-27 A的14 kb区间。虽然这些实验准确地映射了基因，但它们也说明了这种方法的一些复杂性。拯救结果显示，来自该区间的2.8kb基因组片段能够完全拯救皮层表型。2.8kb的拯救片段含有单个开放阅读框架。预测的氨基酸序列表明，皮质编码WD-重复蛋白，是Cdc 20蛋白家族的远距离成员。发育北方分析结果表明，皮质转录本在卵子发生和早期胚胎发生过程中高水平表达。有趣的是，减数分裂中期后期II逮捕缺陷皮质纯合子女性奠定了胚胎类似于有丝分裂中期后期缺陷酵母cdc 20突变体中观察到的。皮质蛋白质的预测性质以及在皮质突变体中观察到的减数分裂表型表明，与cdc 20依赖性APC介导的蛋白水解途径（其控制有丝分裂中的中期-后期转变）类似的途径在调节卵母细胞减数分裂中也是重要的，genesis（C）2001 Wiley-Liss，Inc.
Mutations in cortex and grauzone cause abnormal arrest in Drosophila female meiosis. cortex was mapped to a 14 kb interval in 26F-27A by the male recombination mapping method. While these experiments mapped the gene accurately, they also illustrated some complexities of this method. Rescue results showed that a 2.8 kb genomic fragment from this interval was able to fully rescue the cortex phenotype. The 2.8 kb rescuing fragment contains a single open reading frame. The predicted amino acid sequence indicates that cortex encodes a WD-repeat protein and is a distant member of the Cdc20 protein family. Results from a developmental Northern analysis showed that the cortex transcript is expressed at high levels during oogenesis and early embryogenesis. Interestingly, the meiotic metaphase-anaphase II arrest defect in embryos laid by cortex homozygous females resembles the mitotic metaphase-anaphase defects observed in yeast cdc20 mutants. The predicted nature of the Cortex protein, together with the observed meiotic phenotype in cortex mutants, suggest that a similar pathway to the cdc20 dependent APC-mediated proteolysis pathway, which governs the metaphase-anaphase transition in mitosis, is also important in regulating oocyte meiosis, genesis (C) 2001 Wiley-Liss, Inc.