AGGREGATION OF HYDROXYAPATITE CRYSTALS
AGGREGATION OF HYDROXYAPATITE CRYSTALS
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DOI：
10.1016/0304-4165(76)90150-1
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发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
FLEISCH, H
中科院分区：
文献类型：
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作者：
HANSEN, NM;FELIX, R;FLEISCH, H
研制了一套研究羟基磷灰石晶体聚集的系统。几个因素（Ca 2 + × Ca 2+）的影响是显著的。Pi乘积、Ca ~（2+）/Pi比值、pH和各种物质）。Pb 2+，Zn 2+，Mg 2+和亚甲蓝只有很小的影响;柠檬酸盐抑制聚集。PPi是一种强抑制剂，二膦酸盐乙烷-1-羟基-1，1-二膦酸二钠和二氯亚甲基二膦酸二钠甚至更有效。单膦酸酯pentanemonophosphonate没有效果。糖胺聚糖也出现了有效的抑制作用：肝素>透明质酸>硫酸皮肤素> 4-硫酸软骨素> 6-硫酸软骨素。尿液也显示出较高的抑制活性。白色溶菌酶可消除肝素的抑制作用，而透明质酸、PPi和尿则不能消除。所述效应可能与正常矿化过程和导致病理性钙化（如尿路结石形成）的机制有关。
A system to study the aggregation of hydroxyapatite crystals was developed. The effect of several factors (Ca2+ .times. Pi product, Ca2+/Pi ratio, pH and various substances) were tested. Pb2+, Zn2+, Mg2+ and methyleneblue had only small effects; citrate inhibited aggregation. PPi was a strong inhibitor and the diphosphonates disodium ethane-1-hydroxy-1,1-diphosphonate and disodium dichloromethylene diphosphonate were even more potent. The monophosphonate pentanemonophosphonate had no effect. Potent inhibition also occurred with glycosaminoglycans: heparin > hyaluronic acid > dermatan sulfate > chondroitin 4-sulfate > chondroitin 6-sulfate. Urine also showed high inhibitory activity. The inhibition of heparin but not that of hyaluronic acid, PPi or urine was abolished by egg white lysozyme. The effects described might be relevant in the normal mineralization process and in the mechanisms leading to pathological calcification, such as urinary stone formation.