Single-cell heterogeneity of EGFR and CDK4 co-amplification is linked to immune infiltration in glioblastoma.
Single-cell heterogeneity of EGFR and CDK4 co-amplification is linked to immune infiltration in glioblastoma.
复制标题
DOI：
10.1016/j.celrep.2023.112235
复制
发表时间：
2023-03-28
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
Glioblastoma (GBM) is the most aggressive brain tumor, with a median survival of ~15 months. Targeted approaches have not been successful in this tumor type due to the large extent of intratumor heterogeneity. Mosaic amplification of oncogenes suggests that multiple genetically distinct clones are present in each tumor. To uncover the relationships between genetically diverse subpopulations of GBM cells and their native tumor microenvironment, we employ highly multiplexed spatial protein profiling coupled with single-cell spatial mapping of fluorescence in situ hybridization (FISH) for EGFR, CDK4, and PDGFRA. Single-cell FISH analysis of a total of 35,843 single nuclei reveals that tumors in which amplifications of EGFR and CDK4 more frequently co-occur in the same cell exhibit higher infiltration of CD163+ immunosuppressive macrophages. Our results suggest that high-throughput assessment of genomic alterations at the single-cell level could provide a measure for predicting the immune state of GBM. Glioblastoma cellular diversity in the context of tumor tissue microenvironments remains poorly understood. Walentynowicz et al. use the relative frequency of cells harboring co-amplification of EGFR and CDK4 to classify tumors and show that the presence of genetically distinct subpopulations is associated with differences in immunosuppressive macrophage infiltration in glioblastoma tissue.
登录
查看更多内容
影响因子：
4.6
作者：
Li, Anqi;Bai, Qianming;Yang, Wentao
通讯作者：
Yang, Wentao
影响因子：
30.8
作者：
Chaligne R;Gaiti F;Silverbush D;Schiffman JS;Weisman HR;Kluegel L;Gritsch S;Deochand SD;Gonzalez Castro LN;Richman AR;Klughammer J;Biancalani T;Muus C;Sheridan C;Alonso A;Izzo F;Park J;Rozenblatt-Rosen O;Regev A;Suvà ML;Landau DA
通讯作者：
Landau DA
影响因子：
10.5
作者：
Janiszewska, Michalina;Suva, Mario L.;Stamenkovic, Ivan
通讯作者：
Stamenkovic, Ivan
影响因子：
22.7
作者：
Garofano L;Migliozzi S;Oh YT;D'Angelo F;Najac RD;Ko A;Frangaj B;Caruso FP;Yu K;Yuan J;Zhao W;Di Stefano AL;Bielle F;Jiang T;Sims P;Suvà ML;Tang F;Su XD;Ceccarelli M;Sanson M;Lasorella A;Iavarone A
通讯作者：
Iavarone A
影响因子：
8
作者：
Janiszewska, Michalina;Stein, Shayna;Polyak, Kornelia
通讯作者：
Polyak, Kornelia