The [URE3] prion is an aggregated form of Ure2p that can be cured by overexpression of Ure2p fragments
The [URE3] prion is an aggregated form of Ure2p that can be cured by overexpression of Ure2p fragments
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DOI：
10.1073/pnas.96.4.1498
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发表时间：
1999-02-16
影响因子：
11.1
通讯作者：
Wickner, RB
中科院分区：
文献类型：
--
作者：
Edskes, HK;Gray, VT;Wickner, RB
The [URE3] nonchromosomal genetic element is a prion of Ure2p, a regulator of nitrogen catabolism in Saccharomyces cerevisiae. Ure2p(1-65) is the prion domain of Ure2p, sufficient to propagate [URE3] in vivo. We show that full length Ure2p-green fluorescent protein (GFP) or a Ure2p(1-65)-GFP fusion protein is aggregated in cells carrying [URE3] but is evenly distributed in cells lacking the [URE3] prion. This indicates that [URE3] involves a self-propagating aggregation of Ure2p. Overexpression of Ure2p(1-65) induces the de novo appearance of [URE3] by 1,000-fold in a strain initially [ure-o], but cures [URE3] from a strain initially carrying the [URE3] prion. Overexpression of several other fragments of Ure2p or Ure2-GFP fusion proteins also efficiently cures the prion. We suggest that incorporation of fragments or fusion proteins into a putative [URE3] "crystal" of Ure2p poisons its propagation.
