Immunohistochemical detection of CCR2 and CX3CR1 in sepsis-induced lung injury
Immunohistochemical detection of CCR2 and CX3CR1 in sepsis-induced lung injury
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DOI：
10.1016/j.forsciint.2009.08.007
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发表时间：
2009-11-20
影响因子：
2.2
通讯作者：
Kondo, Toshikazu
中科院分区：
文献类型：
--
作者：
An, Jun-Ling;Ishida, Yuko;Kondo, Toshikazu
脓毒症是一种全身性炎症性疾病，病死率高。在本研究中，我们用免疫组织化学方法检测了CCR2和CX3CR1在脓毒症所致肺损伤中的表达，并探讨其在脓毒症尸检中的应用。从9例脓毒症患者和8例对照组的不同肺叶采集肺组织标本，时间间隔为12~48h，免疫组织化学显示，脓毒症组和非脓毒症组肺组织中均有CCR2和CX3CR1的表达。在双色免疫荧光分析中，CCR2或CX3CR1阳性细胞可被鉴定为CD68阳性的巨噬细胞。CD68阳性的巨噬细胞多数同时表达CCR2和CX3CR1。在形态计量学上，脓毒症组CCR2和CX3CR1阳性巨噬细胞的平均数明显高于对照组(CCR2组为41.6+/-1.3%，对照组为8.0+/-0.4%；CX3CR1组为36.2+/-1.3%，对照组为9.2+/-0.4%)。这些观察表明，在几种病理条件下，CCR2或CX3CR1阳性的巨噬细胞被招募到肺内。特别是，他们的招募可能在脓毒症中更加明显。此外，从法医学角度来看，免疫组织化学检测肺组织中CCR2和CX3CR1的表达可作为身体诊断脓毒症的有价值的工具。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Sepsis is a systemic inflammatory disease with high mortality. In the present study, we immunohistochemically examined CCR2 and CX3CR1 expression in sepsis-induced lung injury, and discussed its availability for the postmortem diagnosis of sepsis. Lung samples were obtained from different lung lobes of nine sepsis and eight control cases with postmortem intervals between 12 and 48 h. Immunohistochemically, mononuclear cells recruited into the lungs expressed CCR2 and CX3CR1 in both sepsis and non-septic groups. In double-color immunofluorescence analysis, CCR2- or CX3CR1-positive cells could be identified as CD68-positive macrophages. Moreover, most of CD68-positive macrophages expressed both CCR2 and CX3CR1. Morphometrically, the average of CCR2- and CX3CR1-positive macrophages was significantly increased in sepsis group, compared with control group ( sepsis vs. control: 41.6 +/- 1.3% vs. 8.0 +/- 0.4% in CCR2; 36.2 +/- 1.3% vs. 9.2 +/- 0.4% in CX3CR1). These observations implied that CCR2- or CX3CR1-positive macrophages were recruited into the lungs under several pathological conditions. In particular, their recruitment might be more evident in sepsis. Moreover, from the forensic aspects, immunohistochemical detection of CCR2 and CX3CR1 expression in the lungs can be considered as valuable diagnostic tools for the postmortem diagnosis of sepsis. (C) 2009 Elsevier Ireland Ltd. All rights reserved.