The Spatiotemporal Control of Osteoblast Cell Growth, Behavior, and Function Dictated by Nanostructured Stainless Steel Artificial Microenvironments.
The Spatiotemporal Control of Osteoblast Cell Growth, Behavior, and Function Dictated by Nanostructured Stainless Steel Artificial Microenvironments.
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DOI：
10.1186/s11671-016-1810-1
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发表时间：
2017-12
影响因子：
--
通讯作者：
Chen WL
中科院分区：
文献类型：
--
作者：
Dhawan U;Pan HA;Shie MJ;Chu YH;Huang GS;Chen PC;Chen WL
纳米结构生物材料作为植入物的成功应用很大程度上取决于触发理想细胞响应的纳米形貌尺寸。在这里，我们设计了304L不锈钢表面的纳米多孔形貌，以识别引起细胞特性转变的纳米形貌尺寸，并据此提出了将纳米结构不锈钢表面作为骨科植入物的设计方案。通过一步电解法制备了直径从100 nm到220 nm的各种纳米孔，统称为人工微环境。通过改变偏置电压实现了对纳米孔直径的控制。将MG63成骨细胞在纳米孔表面培养不同天数。免疫荧光(IF)和扫描电子显微镜(SEM)比较细胞形态和特性的改变。纳米孔达到一定直径后，成骨细胞表现出不同的生长参数和明显的细胞行为转变。直径为100 nm的纳米孔可促进细胞生长、局部粘连、细胞面积、活力、纽蛋白染色面积、钙矿化和碱性磷酸酶活性。这些纳米多孔基质能够以不同的方式调节细胞行为并帮助识别过渡步骤，这将有利于生物医学工程师开发出优越的植入几何结构，在细胞-纳米拓扑学界面触发理想的细胞响应。
The successful application of a nanostructured biomaterial as an implant is strongly determined by the nanotopography size triggering the ideal cell response. Here, nanoporous topography on 304L stainless steel substrates was engineered to identify the nanotopography size causing a transition in the cellular characteristics, and accordingly, the design of nanostructured stainless steel surface as orthopedic implants is proposed. A variety of nanopore diameters ranging from 100 to 220 nm were fabricated by one-step electrolysis process and collectively referred to as artificial microenvironments. Control over the nanopore diameter was achieved by varying bias voltage. MG63 osteoblasts were cultured on the nanoporous surfaces for different days. Immunofluorescence (IF) and scanning electron microscopy (SEM) were performed to compare the modulation in cell morphologies and characteristics. Osteoblasts displayed differential growth parameters and distinct transition in cell behavior after nanopore reached a certain diameter. Nanopores with 100-nm diameter promoted cell growth, focal adhesions, cell area, viability, vinculin-stained area, calcium mineralization, and alkaline phosphatase activity. The ability of these nanoporous substrates to differentially modulate the cell behavior and assist in identifying the transition step will be beneficial to biomedical engineers to develop superior implant geometries, triggering an ideal cell response at the cell-nanotopography interface.