Identification of heavy metal induced changes in the expression patterns of the translationally controlled tumour protein (TCTP) in the earthworm Lumbricus rubellus
Identification of heavy metal induced changes in the expression patterns of the translationally controlled tumour protein (TCTP) in the earthworm Lumbricus rubellus
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DOI：
10.1016/s0167-4781(98)00077-3
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发表时间：
1998-07-09
期刊：
影响因子：
--
通讯作者：
Morgan, AJ
中科院分区：
文献类型：
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作者：
Stürzenbaum, SR;Kille, P;Morgan, AJ
在生物监测过程中，政府监管机构利用丰富的土壤无脊椎动物蚯蚓对重金属污染土壤的特定人类健康和生态风险进行了评估。对重金属暴露引起的分子遗传反应进行指纹鉴定有助于确定生物标志物，以评估这种污染对个体生物体的影响。本文报道了一种新的翻译控制肿瘤蛋白（TCTP）在风疹蚓（Lumbricus rubellus）中的鉴定。除了标准的差异Southern印迹分子生物学技术外，还采用了一种完全定量的方法（荧光微体积PCR）来评估暴露于不同重金属环境的蚯蚓中TCTP的特异性表达谱。在以肌动蛋白作为不变对照归一化后，结果表明，与未受污染的对照人群相比，来自铅/锌/镉污染矿山的人群中TCTP至少上调了4倍。在铜污染的矿井中发现的蚯蚓中，TCTP增加了335倍，增幅更为明显。暴露在具有单一胁迫源（Cd）的人工土壤中的蚯蚓的TCTP拷贝数比适宜对照蚯蚓（在没有Cd的人工土壤中维持）高14倍。提出的数据在两个方面是新颖的：首先，它们为重金属（尤其是铜）诱导的上调提供了证据；其次，他们表明，TCTP也可以受到转录控制，因此，上调并不局限于翻译修饰，正如TCTP的命名所暗示的那样。(C) 1998 Elsevier Science B.V.版权所有
Heavy metal contaminated soils are assessed for specific human health and ecological risk by governmental regulatory agencies utilizing the abundant soil invertebrate, the earthworm, in a biomonitoring process. Fingerprinting the molecular genetic responses resulting from heavy metal exposure facilitates the identification of biomarkers for assessing the impact of such pollution on individual organisms. This paper reports the identification of a novel translationally controlled tumour protein (TCTP) in the earthworm Lumbricus rubellus. In addition to the standard molecular biological technique of differential Southern blotting, a fully quantitative approach (fluorescent microvolume PCR) was performed to assess the specific expression profiles of TCTP in earthworms exposed to different heavy metal regimes. After normalizing with actin as an invariant control, the results showed that TCTP was upregulated by at least a factor of 4 in the population originating from a Pb/Zn/Cd polluted mine, compared to an unpolluted control population. An even more pronounced increase was identified in earthworms native to a Cu polluted mine, where TCTP increased 335-fold. TCTP copies in earthworms exposed to artificial soil with a single stressor (Cd) were 14 times higher than in the appropriate control earthworms (maintained on artificial soil without Cd). The data presented are novel in two ways: first, they provide evidence for an upregulation that is induced by heavy metals (especially copper); second, they show that TCTP can also be under transcriptional control, therefore upregulation is not limited to translational modifications as TCTP's nomenclature suggests. (C) 1998 Elsevier Science B.V. All rights reserved.