Expression of gamma‐glutamyltranspeptidase in cultures of spontaneously and chemically transformed rat liver cells
Expression of gamma‐glutamyltranspeptidase in cultures of spontaneously and chemically transformed rat liver cells
复制标题
自发和化学转化大鼠肝细胞培养物中γ-谷氨酰转肽酶的表达
DOI：
10.1002/ijc.2910320318
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发表时间：
1983
影响因子：
6.4
通讯作者：
Jiro Sato
中科院分区：
文献类型：
--
作者：
M. Miyazaki;S. Wahid;Keiko Miyano;Tamae Yabe;Jiro Sato
研究了γ -谷氨酰转肽酶（γ - GTP）在正常和氨基偶氮染料喂养大鼠肝脏中38株致瘤性和非致瘤性上皮细胞系中的表达及其与致瘤性的关系。22个非致瘤性上皮细胞系中有9个表现出γ - GTP阳性细胞，而16个致瘤性上皮细胞系中有9个不表现出γ - GTP阳性细胞。这一发现表明γ - GTP活性的获得与致瘤性之间不存在相关性。10个自发转化的上皮细胞系，包括8个致瘤性细胞系和2个非致瘤性细胞系，没有一个显示出γ - GTP -阳性细胞。另一方面，来自氨基偶氮染料喂养的大鼠肝脏和氨基偶氮染料处理的正常大鼠肝细胞培养物的28个转化上皮细胞系中，有16个包含不同百分比的γ - GTP阳性细胞。如上所述，与大鼠肝细胞的体外自发转化相比，很明显，致癌物诱导的大鼠肝细胞在体外和体内的转化往往伴随着γ - GTP活性的获得。因此，我们认为肝细胞中γ - GTP的表达可能主要与暴露于化学致癌物有关。
The expression of gamma‐glutamyltranspeptidase (gamma‐GTP) and the relationship between this enzyme activity and tumorigenicity were studied in 38 tumorigenic and non‐tumorigenic epithelial cell lines derived from livers of normal and aminoazo‐dye‐fed rats. Nine of 22 non‐tumorigenic epithelial cell lines exhibited gamma‐GTP‐positive cells, whereas 9 of 16 tumorigenic epithelial cell lines did not exhibit gamma‐GTP‐positive cells. This finding revealed that no correlation existed between the acquisition of gamma‐GTP activity and tumorigenicity. None of 10 spontaneously transformed epithelial cell lines, which consisted of 8 tumorigenic and 2 nontumorigenic cell lines, exhibited gamma‐GTP‐positive cells. On the other hand, 16 of 28 transformed epithelial cell lines, which were derived from aminoazo‐dye‐fed rat livers and aminoazo‐dye‐treated cultures of normal rat liver cells, included gamma‐GTP‐positive cells in various percentages. As described above, in contrast to in vitro spontaneous transformation of rat liver cells, it is obvious that the carcinogen‐induced transformation of rat liver cells in vitro as well as in vivo was frequently accompanied by the acquisition of gamma‐GTP‐activity. Therefore, it is concluded that the expression of gamma‐GTP in liver cells may be mainly associated with exposure to chemical carcinogens.