Lipoprotein is a predominant Toll-like receptor 2 ligand in Staphylococcus aureus cell wall components
Lipoprotein is a predominant Toll-like receptor 2 ligand in Staphylococcus aureus cell wall components
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DOI：
10.1093/intimm/dxh374
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发表时间：
2006-02-01
影响因子：
4.4
通讯作者：
Suda, Y
中科院分区：
文献类型：
--
作者：
Hashimoto, M;Tawaratsumida, K;Suda, Y
据报道，源自金黄色葡萄球菌的脂磷壁酸 (LTA) 是 Toll 样受体 2 (TLR2) 的配体。在这项研究中，我们证明从金黄色葡萄球菌中获得的脂蛋白是 TLR2 的有效激活剂。 Triton X-114 通过 TLR2 相分配活化细胞获得的级分。该级分含有蛋白质和 LTA。在质量范围为 12-40 kDa 的化合物中检测到活性。蛋白酶 K 将活性化合物消化成 < 10 kDa 的低分子量活性物质。相比之下，分解LTA的氢氟酸处理并没有改变活性化合物的分子质量。此外，大部分活性被脂蛋白脂肪酶消化消除。这些结果表明脂蛋白是金黄色葡萄球菌细胞壁成分中主要的 TLR2 配体。
Lipoteichoic acid (LTA) derived from Staphylococcus aureus is reported to be a ligand of Toll-like receptor 2 (TLR2). In this study, we demonstrated that lipoproteins obtained from S. aureus are potent activators of TLR2. A fraction obtained by Triton X-114 phase partitioning activated cells through TLR2. The fraction contained proteins and LTA. The activity was detected in compounds in a mass range of 12-40 kDa. Proteinase K digested the active compounds into lower molecular weight active materials < 10 kDa. In contrast, hydrofluoric acid treatment, which decomposes LTA, did not alter the molecular mass of the active compounds. Further, most of the activity was abrogated by lipoprotein lipase digestion. These results suggested that lipoproteins are predominant TLR2 ligands in S. aureus cell wall components.