The effects of brassinosteroid on the induction of biochemical changes in Lycopersicon esculentum under drought stress
The effects of brassinosteroid on the induction of biochemical changes in Lycopersicon esculentum under drought stress
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DOI：
10.3906/bot-0806-12
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发表时间：
2009-01-01
影响因子：
1.8
通讯作者：
Ziaie, Jalal
中科院分区：
文献类型：
--
作者：
Behnamnia, Mehri;Kalantari, Khosrow Manouchehri;Ziaie, Jalal
干旱胁迫被认为是植物产品的一个限制因素;因此，许多化合物被用于最大限度地减少胁迫的有害影响。具有抗氧化特性的这些化合物的一种类型是油菜素类固醇。在该实验中，当番茄植株出现4片完全展开的叶时，将24-表油菜素（24-EBL）以0.01和1 μ M的浓度喷洒在叶上3天，间隔1天。三个水平的干旱胁迫（0，3，和5天的停水）。在此基础上，研究了油菜素内酯和水分胁迫对番茄生理生化和抗氧化指标的影响。膜脂过氧化、过氧化氢和脯氨酸含量在干旱胁迫下增加。干旱条件下蛋白质含量的减少表明干旱胁迫影响蛋白质的合成和降解。在干旱胁迫下，抗氧化酶（POD和APX）活性下降，SOD、GR和CAT活性升高。24-EBL和干旱胁迫处理的植物中脂质过氧化和H2 O2含量的减少。根据我们的研究结果，似乎油菜素类固醇大大减轻了氧化损伤，发生在干旱胁迫下。BR处理的干旱胁迫植物中抗氧化酶（POD、CAT、APX、GR和SOD）活性的增加和同工酶谱的变化以及脯氨酸和蛋白质含量的增加可能表明油菜素内酯在提高植物对水分胁迫的耐受性中的作用。因此，24-表油菜素可能在减轻由水分胁迫引起的损害中起作用。
Drought stress is considered a restricting factor for plant products; therefore many compounds were applied to minimise the harmful effects of stress. One type of these compounds that have antioxidative characteristics is brassinosteroids. In this experiment, when 4 fully expanded leaves of tomato plants appeared, 24-epibrassinolide (24-EBL) was sprayed onto the leaves, at 0.01 and 1 mu M concentrations for 3 days with a 1-day interval. Three levels of drought stress (0, 3, and 5 days withholding water) were applied. Thereafter, the effects of brassinosteroids and water stress were investigated on some biochemical and antioxidative parameters of tomato plants. Lipid peroxidation, peroxide hydrogen, and proline content increased in plants subjected to drought stress. Reduction in protein content in drought conditions showed that drought stress affected protein synthesis and degradation. Decline in the activity of antioxidant enzymes (POD and APX) and increases in the SOD, GR, and CAT activities were observed in drought stressed plants. Reduction in peroxidation of lipids and H2O2 content in the plants treated with both 24-EBL and drought stress was observed. Based on our results it seems that brassinosteroids considerably alleviated oxidative damage that occurred under drought stress. Increase in activity of antioxidant enzymes (POD, CAT, APX, GR, and SOD) and change in isoenzymes pattern with higher intensity as well as increases in proline and protein content in drought stressed plants treated with BR will probably show the role of brassinosteroids in increased tolerance of plants to water stress. Therefore, 24-epibrassinolide may have a role in the mitigation of damage caused by water stress.