Evidence that griseofulvin binds to a microtubule associated protein
Evidence that griseofulvin binds to a microtubule associated protein
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灰黄霉素与微管相关蛋白结合的证据
DOI：
10.1016/0014-5793(77)80073-2
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发表时间：
1977
期刊：
影响因子：
3.5
通讯作者：
C. I. Pogson
中科院分区：
文献类型：
--
作者：
A. Roobol;K. Gull;C. I. Pogson
我们最近发现抗有丝分裂药物灰黄霉素在体外抑制脑微管的组装[1]。Weber等人也提供了类似的数据。[2]的文件。我们早期的实验表明灰黄霉素通过阻止微管蛋白和微管相关蛋白（MAPS）之间的结合来抑制微管组装，微管相关蛋白通常与微管蛋白共纯化[3]并促进纯化的微管蛋白二聚体的组装[4]。我们现在提出的证据表明，灰黄霉素特异性结合MAPS，而不是微管蛋白二聚体的浓度灰黄霉素抑制微管组装。
We have recently shown that the anti-mitotic drug, griseofulvin, inhibits assembly of brain microtubules in vitro [l]. Similar data have been presented by Weber et al.[2]. Our earlier experiments indicated that griseofulvin inhibits microtubule assembly by preventing the association between tubulin and the microtubule associated proteins (MAPS) which normally co-purify with tubulin [3] and promote assembly of purified tubulin dimer [4]. We now present evidence that griseofulvin binds specifically to MAPS and not to tubulin dimer at concentrations of griseofulvin which inhibits microtubule assembly.