STRUCTURAL AND FUNCTIONAL CONSERVATION OF 2 HUMAN HOMOLOGS OF THE YEAST DNA-REPAIR GENE RAD6
STRUCTURAL AND FUNCTIONAL CONSERVATION OF 2 HUMAN HOMOLOGS OF THE YEAST DNA-REPAIR GENE RAD6
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DOI：
10.1073/pnas.88.20.8865
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发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
HOEIJMAKERS, JHJ
中科院分区：
文献类型：
--
作者：
KOKEN, MHM;REYNOLDS, P;HOEIJMAKERS, JHJ
酿酒酵母的RAD6基因编码DNA修复，损伤诱导的诱变和孢子形成所需的泛素偶联酶（E2）。我们将两个人的RAD6同源物克隆为指定的HHR6A和HHR6B。两种152个氨基酸人蛋白相互共享95％的序列认同，几乎等于70％，几乎等于酵母（酿酒酵母和精神分裂症的同源物）的总体身份几乎等于85％。 ， 分别。酿酒酵母rad6蛋白中的酸性C末端序列均未均不具有酸性的C末端序列。遗传互补实验表明，HHR6A以及HHR6B可以在酿酒酵母rad6-二尔达氏菌突变体中执行RAD6的DNA修复和诱变功能。
The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme (E2) that is required for DNA repair, damage-induced mutagenesis, and sporulation. We have cloned the two human RAD6 homologs, designated HHR6A and HHR6B. The two 152-amino acid human proteins share 95% sequence identity with each other and almost-equal-to 70% and almost-equal-to 85% overall identity with the homologs from yeasts (S. cerevisiae and Schizosaccharomyces pombe) and Drosophila melanogaster, respectively. Neither of the human RAD6 homologs possesses the acidic C-terminal sequence present in the S. cerevisiae RAD6 protein. Genetic complementation experiments reveal that HHR6A as well as HHR6B can carry out the DNA repair and mutagenesis functions of RAD6 in S. cerevisiae rad6-DELTA mutants.