Gastric juice piR-1245: A promising prognostic biomarker for gastric cancer
Gastric juice piR-1245: A promising prognostic biomarker for gastric cancer
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胃液 piR-1245：一种有前景的胃癌预后生物标志物
DOI：
10.1002/jcla.23131
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发表时间：
2020-04-01
影响因子：
2.7
通讯作者：
Lu, Jianwei
中科院分区：
文献类型：
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作者：
Zhou, Xiaorong;Liu, Jianhong;Lu, Jianwei
研究背景近年来的研究表明，PIWI相互作用RNA（piRNA）在肿瘤发生中起着不可或缺的作用。然而，胃特异性胃液中的piR-1245是否可用作胃癌（GC）的生物标志物仍然是未知的。为探讨piR-1245在胃癌诊断和预后判断中的应用价值，本研究收集了66例胃癌患者和66例健康人的胃液，并对piR-1245在胃癌诊断和预后判断中的作用进行了研究。采用定量实时逆转录酶聚合酶链反应（qRT-PCR）测量piR-1245表达水平。然后，在GC患者和健康个体之间确定胃液中piR-1245表达的模式。结果胃癌患者胃液piR-1245水平显著高于正常对照组（P <0.0001），胃癌患者胃液piR-1245水平显著高于正常对照组（P <0.0001）。ROC曲线下面积（AUC）为0.885（敏感性90.9%，特异性74.2%，95%可信区间0.8286 ~ 0.9414）。高胃液piR-1245表达与肿瘤大小（P = 0.013）和TNM分期（P = 0.001）显著相关。在胃液中具有高piR-1245表达的GC患者表现出较差的总生存期（OS）（P = 0.0152）和无进展生存期（PFS）（P = 0.013）。考克斯回归分析证实，胃液piR-1245表达是胃癌患者生存期的独立危险因素（P < .05）。
Background Emerging reports demonstrated that PIWI-interacting RNAs (piRNAs) played an indispensable role in tumorigenesis. However, it still remains elusive whether piR-1245 in gastric juice specific in stomach could be employed as a biomarker for gastric cancer (GC). The present work is aiming at exploring the possibility of piR-1245 in gastric juice as a potential marker to judge for diagnosis and prognosis of gastric cancer.Methods Gastric juice was collected from 66 GC patients and 66 healthy individuals. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was employed to measure the levels of piR-1245 expression. Then, the pattern of piR-1245 expression in gastric juice was determined between GC patients and healthy individuals. A receiver operating characteristic (ROC) curve was constructed for distinguishing GC from healthy individuals.Results Gastric juice piR-1245 levels in GC were higher than those of controls (P < .0001). The value of area under ROC (AUC) was 0.885 (sensitivity, 90.9%; specificity, 74.2%; 95% confidence interval, 0.8286 to 0.9414). High gastric juice piR-1245 expression was signally correlated with tumor size (P = .013) and TNM stage (P = .001). GC patients with high piR-1245 expression in gastric juice exerted a poorer overall survival (OS) (P = .0152) and progression-free survival (PFS) (P = .013). COX regression analysis verified that gastric juice piR-1245 expression was an independent prognostic risk variable for OS (P < .05).Conclusions The current study suggested that piR-1245 in gastric juice had the potential to be a useful biomarker for GC detection and prognosis prediction.