magp4 gene may contribute to the diversification of cichlid morphs and their speciation
magp4 gene may contribute to the diversification of cichlid morphs and their speciation
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DOI：
10.1016/j.gene.2006.01.016
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发表时间：
2006-05-24
期刊：
影响因子：
3.5
通讯作者：
Okada, Norihiro
中科院分区：
文献类型：
--
作者：
Kobayashi, Naoki;Watanabe, Masakatsu;Okada, Norihiro
维多利亚湖拥有300多种慈鲷，它们适应于各种生态位，具有各种形态学物种特异性特征。然而，人们认为这些物种在过去的14,000年里爆发性地出现，维多利亚湖慈鲷物种之间的转录本在序列上几乎是相同的。这些数据促使我们开发了一种DNA芯片检测方法，以比较慈鲷属物种之间的基因表达模式。我们准备了一个DNA芯片斑点与6240个元素来自慈鲷表达序列标签（EST）克隆，并成功地表征慈鲷物种Haplochromis chilotes和Haplochromis sp.“rockkribensis”之间的基因表达差异。我们确定了14个转录本，这些物种之间的差异表达在早期发育阶段，受精后15天（dpf），并进一步分析了几个使用定量实时PCR（qPCR）。这些差异表达的转录本之一是微原纤维相关糖蛋白4（magp 4）的同源物，这是人类遗传性疾病Smith-Magenis综合征（SMS）的假定致病基因，面部缺陷是其表型特征之一。进一步的magp 4表达分析表明，magp 4在慈鲷鱼苗的下颚部分表达，并在发育过程中的表达谱Haplochromis chilotes和Haplochromis sp.“rockkribensis”之间存在差异。这些数据表明，与人类颅骨形态发生相关的基因的差异表达可能参与了慈鲷颌骨形态的多样化。(c)2006 Elsevier B. V.保留所有权利。
Lake Victoria harbors more than 300 species of cichlid fish, which are adapted to a variety of ecological niches with various morphological species-specific features. However, it is believed that these species arose explosively within the last 14,000 years and transcripts among Lake Victoria cichlid species are almost identical in sequence. These data prompted us to develop a DNA chip assay to compare patterns of gene expression among cichlid species. We prepared a DNA chip spotted with 6240 elements derived from cichlid expressed sequence tag (EST) clones and successfully characterized gene expression differences between the cichlid species Haplochromis chilotes and Haplochromis sp. "rockkribensis". We identified 14 transcripts that were differentially expressed between these species at an early developmental stage, 15 days post-fertilization (dpf), and several were further analyzed using quantitative real-time PCR (qPCR). One of these differentially expressed transcripts was a homolog of microfibril-associated glycoprotein 4 (magp4), a putative causative gene for the human inherited disease, Smith-Magenis syndrome (SMS), for which facial defects are among the phenotypic features. Further analysis of magp4 expression showed that magp4 was expressed in the jaw portion of cichlid fry and that expression profiles between Haplochromis chilotes and Haplochromis sp. "rockkribensis" differed during development. These data suggest that the differential expression of a gene associated with human cranial morphogenesis may be involved in the diversification of cichlid jaw morphs. (c) 2006 Elsevier B.V. All rights reserved.