Scanning electron microscopy of cell surface antigens labeled with colloidal gold.
Scanning electron microscopy of cell surface antigens labeled with colloidal gold.
复制标题
胶体金标记的细胞表面抗原的扫描电子显微镜。
DOI：
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发表时间：
1986
影响因子：
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通讯作者：
Davide Soligo
中科院分区：
文献类型：
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作者：
E. D. harven;Davide Soligo
描述并讨论了一种方法，该方法允许用扫描电子显微镜观察的胶体金标记物对前置细胞的表面进行特定标记。它的价值完全取决于背向散射电子成像的使用。它的优点包括可能保留标记细胞的表面特征，易于建立特异性，可能对标记的表面抗原位置进行总计数，以及可能实现对同一细胞表面表达的两种不同抗原的不同标记。
A method is described and discussed that permits the specific labeling of the surface of prefixed cells with the colloidal gold marker viewed with the scanning electron microscope. Its value depends exclusively on the use of backscattered electron imaging. Its advantages include the possibility of preserving the surface features of the labeled cells, the ease with which specificity can be established, the possibility of making total counts of the labeled surface antigenic sites, and the possibility of achieving distinct labeling for two different antigens expressed on the surface of the same cell.